TY - JOUR
T1 - Noninvasive monitoring of environmental toxicity through green fluorescent protein expressing Escherichia coli
AU - Song, Young Hoon
AU - Kim, Chang Sup
AU - Seo, Jeong Hyun
N1 - Publisher Copyright:
© 2016, Korean Institute of Chemical Engineers, Seoul, Korea.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - The facile and rapid monitoring of the cellular response to environmental stresses is crucial for understanding the effects of environmental toxicity in living organisms. The overall cell growth can be examined to find a simple monitoring system. Green fluorescent protein (GFP) is advantageous when used as a reporter protein of the cellular stress responses in Escherichia coli because it allows the non-invasive monitoring of GFP in vivo without affecting the cell metabolism. We compared the environmental toxicities of chemical pollutants using GFP expressing E. coli for easy monitoring by incubation in various concentrations of harmful chemicals (ethanol, phenol, para-formaldehyde, paraben, and triclosan). The results showed that all the chemical pollutants act on cell growth and the cell metabolism according to the measured cell density and fluorescent intensity of GFP. In addition, from comparative analysis for quantification, the concentration of unknown ethanol toxicity, which was not determined at that concentration, could be deduced. In conclusion, the degree of toxicity for each chemical pollutant could be estimated or evaluated. This system will be useful for monitoring the toxicity of chemical pollutants in a non-invasive monitoring system.
AB - The facile and rapid monitoring of the cellular response to environmental stresses is crucial for understanding the effects of environmental toxicity in living organisms. The overall cell growth can be examined to find a simple monitoring system. Green fluorescent protein (GFP) is advantageous when used as a reporter protein of the cellular stress responses in Escherichia coli because it allows the non-invasive monitoring of GFP in vivo without affecting the cell metabolism. We compared the environmental toxicities of chemical pollutants using GFP expressing E. coli for easy monitoring by incubation in various concentrations of harmful chemicals (ethanol, phenol, para-formaldehyde, paraben, and triclosan). The results showed that all the chemical pollutants act on cell growth and the cell metabolism according to the measured cell density and fluorescent intensity of GFP. In addition, from comparative analysis for quantification, the concentration of unknown ethanol toxicity, which was not determined at that concentration, could be deduced. In conclusion, the degree of toxicity for each chemical pollutant could be estimated or evaluated. This system will be useful for monitoring the toxicity of chemical pollutants in a non-invasive monitoring system.
KW - Chemical Pollutants
KW - Escherichia coli (E. coli)
KW - Green Fluorescent Protein (GFP)
KW - Noninvasive Monitoring
KW - Stress
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=84955243943&partnerID=8YFLogxK
U2 - 10.1007/s11814-015-0253-1
DO - 10.1007/s11814-015-0253-1
M3 - Article
AN - SCOPUS:84955243943
SN - 0256-1115
VL - 33
SP - 1331
EP - 1336
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
IS - 4
ER -