Abstract
(Chemical Equation Presented) Serving a double purpose: 3′-O-fluorophore-labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′-O-fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing-by-synthesis technology.
Original language | English |
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Pages (from-to) | 75-78 |
Number of pages | 4 |
Journal | ChemBioChem |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - 4 Jan 2010 |
Keywords
- DNA
- Fluorescence
- Next-generation sequencing
- Nucleotides
- Reversible terminator
- Sequencing-by-synthesis