Novel 3′-O-fluorescently modified nucleotides for reversible termination of DNA synthesis

Taek Soo Kim, Da Rae Kim, Hee Chul Ahn, Dongyun Shin, Dae Ro Ahn

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

(Chemical Equation Presented) Serving a double purpose: 3′-O-fluorophore-labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′-O-fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing-by-synthesis technology.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalChemBioChem
Volume11
Issue number1
DOIs
StatePublished - 4 Jan 2010

Keywords

  • DNA
  • Fluorescence
  • Next-generation sequencing
  • Nucleotides
  • Reversible terminator
  • Sequencing-by-synthesis

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