Novel 3′-O-fluorescently modified nucleotides for reversible termination of DNA synthesis

Taek Soo Kim; Da Rae Kim; Hee Chul Ahn; Dongyun Shin; Dae Ro Ahn

doi:10.1002/cbic.200900632

Novel 3′-O-fluorescently modified nucleotides for reversible termination of DNA synthesis

Taek Soo Kim
, Da Rae Kim
, Hee Chul Ahn
, Dongyun Shin
, Dae Ro Ahn

Department of Pharmacy

Korea Institute of Science and Technology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

(Chemical Equation Presented) Serving a double purpose: 3′-O-fluorophore-labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′-O-fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing-by-synthesis technology.

Original language	English
Pages (from-to)	75-78
Number of pages	4
Journal	ChemBioChem
Volume	11
Issue number	1
DOIs	https://doi.org/10.1002/cbic.200900632
State	Published - 4 Jan 2010

Keywords

DNA
Fluorescence
Next-generation sequencing
Nucleotides
Reversible terminator
Sequencing-by-synthesis

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10.1002/cbic.200900632

Cite this

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title = "Novel 3′-O-fluorescently modified nucleotides for reversible termination of DNA synthesis",

abstract = "(Chemical Equation Presented) Serving a double purpose: 3′-O-fluorophore-labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′-O-fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing-by-synthesis technology.",

keywords = "DNA, Fluorescence, Next-generation sequencing, Nucleotides, Reversible terminator, Sequencing-by-synthesis",

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AU - Kim, Taek Soo

AU - Kim, Da Rae

AU - Ahn, Hee Chul

AU - Shin, Dongyun

AU - Ahn, Dae Ro

PY - 2010/1/4

Y1 - 2010/1/4

N2 - (Chemical Equation Presented) Serving a double purpose: 3′-O-fluorophore-labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′-O-fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing-by-synthesis technology.

AB - (Chemical Equation Presented) Serving a double purpose: 3′-O-fluorophore-labeled dTTPs were synthesized and used as reversible terminators of DNA polymerization. Successful incorporation of nucleotides with a bulky 3′-O-fluorophore and detection of fluorescence for base calling can potentially be used to improve sequencing-by-synthesis technology.

KW - DNA

KW - Fluorescence

KW - Next-generation sequencing

KW - Nucleotides

KW - Reversible terminator

KW - Sequencing-by-synthesis

UR - https://www.scopus.com/pages/publications/75649111953

U2 - 10.1002/cbic.200900632

DO - 10.1002/cbic.200900632

M3 - Article

C2 - 19904795

AN - SCOPUS:75649111953

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ER -

Novel 3′-O-fluorescently modified nucleotides for reversible termination of DNA synthesis

Abstract

Keywords

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