Optical stimulation of cultured neurons in 3D microfluidic device coupled to planar microelectrode-arrays

Jae Myung Jang, Sangcheol Na, Seokyoung Bang, Noo Li Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Here, we describe a novel microfluidic system for the optical stimulation of 3D in-vitro neurons. The system was designed to stimulate optically 3D neurons and to record electrically their activity using electrode-arrays measurement. The microfluidic technique enabled to maintain 3D hydrogel structure on the MEAs. We stimulated 3D neurons using the visible light with the commercial caged glutamate. We explored optimum visible light parameters to evoke action potentials in 3D neurons, compared to in 2D neurons. We expect that this technique should open up a new possibility of neural stimulation study in general as well as be utilized for another optical stimulation.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1283-1286
Number of pages4
ISBN (Electronic)9780979806483
StatePublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/TerritoryKorea, Republic of
CityGyeongju
Period25/10/1529/10/15

Keywords

  • 3D neuron
  • Matrigel
  • Micro-electrode arrays
  • Optical stimulation

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