TY - JOUR
T1 - Oxidative stress response in dye degrading bacterium Lysinibacillus sp. RGS exposed to Reactive Orange 16, degradation of RO16 and evaluation of toxicity
AU - Bedekar, Priyanka A.
AU - Saratale, Rijuta G.
AU - Saratale, Ganesh D.
AU - Govindwar, Sanjay P.
N1 - Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Lysinibacillus sp. RGS degrades sulfonated azo dye Reactive Orange 16 (RO16) efficiently. Superoxide dismutase and catalase activity were tested to study the response of Lysinibacillus sp. RGS to the oxidative stress generated by RO16. The results demonstrated that oxidative stress enzymes not only protect the cell from oxidative stress but also has a probable role in decolorization along with an involvement of oxidoreductive enzymes. Formation of three different metabolites after degradation of RO16 has been confirmed by GC-MS analysis. FTIR analysis verified the degradation of functional groups of RO16, and HPTLC confirmed the removal of auxochrome group from the RO16 after degradation. Toxicity studies confirmed the genotoxic, cytotoxic, and phytotoxic nature of RO16 and the formation of less toxic products after the treatment of Lysinibacillus sp. RGS. Therefore, Lysinibacillus sp. RGS has a better perspective of bioremediation for textile wastewater treatment.
AB - Lysinibacillus sp. RGS degrades sulfonated azo dye Reactive Orange 16 (RO16) efficiently. Superoxide dismutase and catalase activity were tested to study the response of Lysinibacillus sp. RGS to the oxidative stress generated by RO16. The results demonstrated that oxidative stress enzymes not only protect the cell from oxidative stress but also has a probable role in decolorization along with an involvement of oxidoreductive enzymes. Formation of three different metabolites after degradation of RO16 has been confirmed by GC-MS analysis. FTIR analysis verified the degradation of functional groups of RO16, and HPTLC confirmed the removal of auxochrome group from the RO16 after degradation. Toxicity studies confirmed the genotoxic, cytotoxic, and phytotoxic nature of RO16 and the formation of less toxic products after the treatment of Lysinibacillus sp. RGS. Therefore, Lysinibacillus sp. RGS has a better perspective of bioremediation for textile wastewater treatment.
KW - Catalase
KW - Cytotoxicity
KW - Decolorization
KW - Genotoxicity
KW - Lysinibacillus sp. RGS
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=84907861126&partnerID=8YFLogxK
U2 - 10.1007/s11356-014-3041-2
DO - 10.1007/s11356-014-3041-2
M3 - Article
C2 - 24888611
AN - SCOPUS:84907861126
SN - 0944-1344
VL - 21
SP - 11075
EP - 11085
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 18
ER -