Oxidative stress response in dye degrading bacterium Lysinibacillus sp. RGS exposed to Reactive Orange 16, degradation of RO16 and evaluation of toxicity

Priyanka A. Bedekar, Rijuta G. Saratale, Ganesh D. Saratale, Sanjay P. Govindwar

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Lysinibacillus sp. RGS degrades sulfonated azo dye Reactive Orange 16 (RO16) efficiently. Superoxide dismutase and catalase activity were tested to study the response of Lysinibacillus sp. RGS to the oxidative stress generated by RO16. The results demonstrated that oxidative stress enzymes not only protect the cell from oxidative stress but also has a probable role in decolorization along with an involvement of oxidoreductive enzymes. Formation of three different metabolites after degradation of RO16 has been confirmed by GC-MS analysis. FTIR analysis verified the degradation of functional groups of RO16, and HPTLC confirmed the removal of auxochrome group from the RO16 after degradation. Toxicity studies confirmed the genotoxic, cytotoxic, and phytotoxic nature of RO16 and the formation of less toxic products after the treatment of Lysinibacillus sp. RGS. Therefore, Lysinibacillus sp. RGS has a better perspective of bioremediation for textile wastewater treatment.

Original languageEnglish
Pages (from-to)11075-11085
Number of pages11
JournalEnvironmental Science and Pollution Research
Volume21
Issue number18
DOIs
StatePublished - 1 Aug 2014

Keywords

  • Catalase
  • Cytotoxicity
  • Decolorization
  • Genotoxicity
  • Lysinibacillus sp. RGS
  • Superoxide dismutase

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