Photo-triggered fluorescent labelling of recombinant proteins in live cells

Deokho Jung; Kohei Sato; Kyoungmi Min; Akira Shigenaga; Juyeon Jung; Akira Otaka; Youngeun Kwon

doi:10.1039/c5cc01067e

Photo-triggered fluorescent labelling of recombinant proteins in live cells

Deokho Jung
, Kohei Sato
, Kyoungmi Min
, Akira Shigenaga
, Juyeon Jung
, Akira Otaka
, Youngeun Kwon

Department of Biomedical Engineering

Dongguk University
Tokushima University
Korea Research Institute of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

Original language	English
Pages (from-to)	9670-9673
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	47
DOIs	https://doi.org/10.1039/c5cc01067e
State	Published - 5 May 2015

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10.1039/c5cc01067e

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title = "Photo-triggered fluorescent labelling of recombinant proteins in live cells",

abstract = "A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.",

author = "Deokho Jung and Kohei Sato and Kyoungmi Min and Akira Shigenaga and Juyeon Jung and Akira Otaka and Youngeun Kwon",

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year = "2015",

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T1 - Photo-triggered fluorescent labelling of recombinant proteins in live cells

AU - Jung, Deokho

AU - Sato, Kohei

AU - Min, Kyoungmi

AU - Shigenaga, Akira

AU - Jung, Juyeon

AU - Otaka, Akira

AU - Kwon, Youngeun

PY - 2015/5/5

Y1 - 2015/5/5

N2 - A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

AB - A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

UR - https://www.scopus.com/pages/publications/84943316739

U2 - 10.1039/c5cc01067e

DO - 10.1039/c5cc01067e

M3 - Article

C2 - 25977944

AN - SCOPUS:84943316739

SN - 1359-7345

VL - 51

SP - 9670

EP - 9673

JO - Chemical Communications

JF - Chemical Communications

IS - 47

ER -

Photo-triggered fluorescent labelling of recombinant proteins in live cells

Abstract

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