Abstract
(Figure Presented) Lightly spliced inteins: A novel strategy to modulate the assembly and trans-splicing activity of the Ssp DnaE split intein was achieved by introducing two photolabile protecting groups onto the backbone of the C-intein polypeptide. This modification was not only able to efficiently block the trans-splicing activity, but also reduce significantly the binding affinity constant between the Cand N-intein fragments. The original activity of the wildtype split intein could be fully recovered by brief exposure to UV light.
Original language | English |
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Pages (from-to) | 1368-1372 |
Number of pages | 5 |
Journal | ChemBioChem |
Volume | 11 |
Issue number | 10 |
DOIs | |
State | Published - 5 Jul 2010 |
Keywords
- Biotechnology
- Peptides
- Photolysis
- Proteins
- Recognition