TY - JOUR
T1 - Physicochemical Properties in 3D Hydrogel Modulate Cellular Reprogramming into Induced Pluripotent Stem Cells
AU - Kim, Deogil
AU - Cha, Byung Hyun
AU - Ahn, Jinsung
AU - Arai, Yoshie
AU - Choi, Bogyu
AU - Lee, Soo Hong
N1 - Publisher Copyright:
© 2020 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH
PY - 2021/2/10
Y1 - 2021/2/10
N2 - Understanding the biophysical relationships between stem cells and applied biomaterials can facilitate the ability to control the functions and behaviors of stem cells. However, the role of 3D microenvironment in stem cell biology remains largely unexplored, compared with that of 2D cell-culture environment. Here, a new strategy that improves the efficacy of Yamanaka's four-factor-induced cellular reprogramming into induced pluripotent stem cells (iPSCs) by incorporating cues derived from the 3D microenvironment and biophysical ligands is reported. Among the various 3D hydrogel systems tested, methacrylated hyaluronic acid (HA) hydrogel significantly improves cellular reprogramming into iPSCs. Additionally, the initial upregulation of CD44 in encapsulated cells in low-level methacrylated soft HA hydrogel accelerates the reprogramming. In conclusion, the reported HA hydrogel with low modulus accelerates reprogramming into iPSCs and thus offers potential advantages for translational applications.
AB - Understanding the biophysical relationships between stem cells and applied biomaterials can facilitate the ability to control the functions and behaviors of stem cells. However, the role of 3D microenvironment in stem cell biology remains largely unexplored, compared with that of 2D cell-culture environment. Here, a new strategy that improves the efficacy of Yamanaka's four-factor-induced cellular reprogramming into induced pluripotent stem cells (iPSCs) by incorporating cues derived from the 3D microenvironment and biophysical ligands is reported. Among the various 3D hydrogel systems tested, methacrylated hyaluronic acid (HA) hydrogel significantly improves cellular reprogramming into iPSCs. Additionally, the initial upregulation of CD44 in encapsulated cells in low-level methacrylated soft HA hydrogel accelerates the reprogramming. In conclusion, the reported HA hydrogel with low modulus accelerates reprogramming into iPSCs and thus offers potential advantages for translational applications.
KW - cellular reprogramming
KW - induced pluripotent stem cells
KW - mesenchymal-epithelial transition
KW - physicochemical 3D microenvironment
UR - http://www.scopus.com/inward/record.url?scp=85096635493&partnerID=8YFLogxK
U2 - 10.1002/adfm.202007041
DO - 10.1002/adfm.202007041
M3 - Article
AN - SCOPUS:85096635493
SN - 1616-301X
VL - 31
JO - Advanced Functional Materials
JF - Advanced Functional Materials
IS - 7
M1 - 2007041
ER -