Physicochemical Properties in 3D Hydrogel Modulate Cellular Reprogramming into Induced Pluripotent Stem Cells

Deogil Kim, Byung Hyun Cha, Jinsung Ahn, Yoshie Arai, Bogyu Choi, Soo Hong Lee

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Understanding the biophysical relationships between stem cells and applied biomaterials can facilitate the ability to control the functions and behaviors of stem cells. However, the role of 3D microenvironment in stem cell biology remains largely unexplored, compared with that of 2D cell-culture environment. Here, a new strategy that improves the efficacy of Yamanaka's four-factor-induced cellular reprogramming into induced pluripotent stem cells (iPSCs) by incorporating cues derived from the 3D microenvironment and biophysical ligands is reported. Among the various 3D hydrogel systems tested, methacrylated hyaluronic acid (HA) hydrogel significantly improves cellular reprogramming into iPSCs. Additionally, the initial upregulation of CD44 in encapsulated cells in low-level methacrylated soft HA hydrogel accelerates the reprogramming. In conclusion, the reported HA hydrogel with low modulus accelerates reprogramming into iPSCs and thus offers potential advantages for translational applications.

Original languageEnglish
Article number2007041
JournalAdvanced Functional Materials
Volume31
Issue number7
DOIs
StatePublished - 10 Feb 2021

Keywords

  • cellular reprogramming
  • induced pluripotent stem cells
  • mesenchymal-epithelial transition
  • physicochemical 3D microenvironment

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