TY - JOUR
T1 - Pontibacter chitinilyticus sp. nov., a novel chitin-hydrolysing bacterium isolated from soil
AU - Chhetri, Geeta
AU - Kim, Jiyoun
AU - Kim, Inhyup
AU - Kim, Myung Kyum
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2019, Springer Nature Switzerland AG.
PY - 2019/7/15
Y1 - 2019/7/15
N2 - A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women’s University field. The strain can grow at 7–37 °C, pH 6.0–8.0, and can tolerate up to 5.5% (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter. The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B). The predominant menaquinone was identified as MK-7. The DNA G+C content was determined to be 48.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp. nov. is proposed. The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).
AB - A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women’s University field. The strain can grow at 7–37 °C, pH 6.0–8.0, and can tolerate up to 5.5% (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter. The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B). The predominant menaquinone was identified as MK-7. The DNA G+C content was determined to be 48.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp. nov. is proposed. The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).
KW - Chitin hydrolysis
KW - Cytophagaceae
KW - Polyphasic taxonomy
KW - Pontibacter chitinilyticus sp. nov
KW - Soil bacteria
UR - http://www.scopus.com/inward/record.url?scp=85061177351&partnerID=8YFLogxK
U2 - 10.1007/s10482-019-01235-1
DO - 10.1007/s10482-019-01235-1
M3 - Article
C2 - 30715624
AN - SCOPUS:85061177351
SN - 0003-6072
VL - 112
SP - 1011
EP - 1018
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 7
ER -