Abstract
For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.
Original language | English |
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Pages (from-to) | 65-72 |
Number of pages | 8 |
Journal | Methods in Molecular Biology |
Volume | 1212 |
DOIs | |
State | Published - 2015 |
Keywords
- Co-culture
- Expansion
- HPSCs
- Porous membrane
- Proliferative human feeder cell
- Self-renewal