Porous membrane culture method for expansion of human pluripotent stem cells

Jin Su Kim, Seung Taeh Hwang, Soo Hong Lee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalMethods in Molecular Biology
Volume1212
DOIs
StatePublished - 2015

Keywords

  • Co-culture
  • Expansion
  • HPSCs
  • Porous membrane
  • Proliferative human feeder cell
  • Self-renewal

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