Porous membrane culture method for expansion of human pluripotent stem cells

Jin Su Kim; Seung Taeh Hwang; Soo Hong Lee

doi:10.1007/7651_2014_174

Porous membrane culture method for expansion of human pluripotent stem cells

Jin Su Kim
, Seung Taeh Hwang
, Soo Hong Lee

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.

Original language	English
Pages (from-to)	65-72
Number of pages	8
Journal	Methods in Molecular Biology
Volume	1212
DOIs	https://doi.org/10.1007/7651_2014_174
State	Published - 2015

Keywords

Co-culture
Expansion
HPSCs
Porous membrane
Proliferative human feeder cell
Self-renewal

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10.1007/7651_2014_174

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title = "Porous membrane culture method for expansion of human pluripotent stem cells",

abstract = "For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.",

keywords = "Co-culture, Expansion, HPSCs, Porous membrane, Proliferative human feeder cell, Self-renewal",

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AU - Lee, Soo Hong

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PY - 2015

Y1 - 2015

N2 - For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.

AB - For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.

KW - Co-culture

KW - Expansion

KW - HPSCs

KW - Porous membrane

KW - Proliferative human feeder cell

KW - Self-renewal

UR - https://www.scopus.com/pages/publications/84929627843

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DO - 10.1007/7651_2014_174

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AN - SCOPUS:84929627843

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VL - 1212

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Porous membrane culture method for expansion of human pluripotent stem cells

Abstract

Keywords

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