TY - JOUR
T1 - Porphyromonas gingivalis-derived lipopolysaccharide-mediated activation of MAPK signaling regulates inflammatory response and differentiation in human periodontal ligament fibroblasts
AU - Seo, Taegun
AU - Cha, Seho
AU - Kim, Tae Il
AU - Lee, Jeong Soon
AU - Woo, Kyung Mi
PY - 2012/4
Y1 - 2012/4
N2 - Porphyromonas gingivalis (P. g.), which is a potential pathogen for periodontal diseases, contains lipopolysaccharide (LPS), and this endotoxin stimulates a variety of cellular responses. At present, P. g.-derived LPS-induced cellular responses in human periodontal ligament fibroblasts (PDLFs) are not well characterized. Here, we demonstrate that P. g-derived LPS regulates inflammatory responses, apoptosis and differentiation in PDLFs. Interleukin-6 (IL-6) and -8 (IL-8) were effectively upregulated by treatment of P. g.-derived LPS, and we confirmed apoptosis markers including elevated cytochrome c levels, active caspase-3 and morphological change in the presence of P. g.-derived LPS. Moreover, when PDLFs were cultured with differentiation media, P. g.-derived LPS reduced the expression of differentiation marker genes, as well as reducing alkaline phosphatase (ALP) activity and mineralization. P. g.-derived LPS-mediated these cellular responses were effectively abolished by treatment of mitogen-activated protein kinase (MAPK) inhibitors. Taken together, our results suggest that P. g.-derived LPS regulates several cellular responses via activation of MAPK signaling pathways in PDLFs.
AB - Porphyromonas gingivalis (P. g.), which is a potential pathogen for periodontal diseases, contains lipopolysaccharide (LPS), and this endotoxin stimulates a variety of cellular responses. At present, P. g.-derived LPS-induced cellular responses in human periodontal ligament fibroblasts (PDLFs) are not well characterized. Here, we demonstrate that P. g-derived LPS regulates inflammatory responses, apoptosis and differentiation in PDLFs. Interleukin-6 (IL-6) and -8 (IL-8) were effectively upregulated by treatment of P. g.-derived LPS, and we confirmed apoptosis markers including elevated cytochrome c levels, active caspase-3 and morphological change in the presence of P. g.-derived LPS. Moreover, when PDLFs were cultured with differentiation media, P. g.-derived LPS reduced the expression of differentiation marker genes, as well as reducing alkaline phosphatase (ALP) activity and mineralization. P. g.-derived LPS-mediated these cellular responses were effectively abolished by treatment of mitogen-activated protein kinase (MAPK) inhibitors. Taken together, our results suggest that P. g.-derived LPS regulates several cellular responses via activation of MAPK signaling pathways in PDLFs.
KW - apoptosis
KW - cell differentiation
KW - lipopolysaccharide
KW - mitogen-activated protein kinase
KW - periodontal ligament
KW - Porphyromonas gingivalis
UR - http://www.scopus.com/inward/record.url?scp=84860361655&partnerID=8YFLogxK
U2 - 10.1007/s12275-012-2146-x
DO - 10.1007/s12275-012-2146-x
M3 - Article
C2 - 22538661
AN - SCOPUS:84860361655
SN - 1225-8873
VL - 50
SP - 311
EP - 319
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 2
ER -