TY - GEN
T1 - Priming via three-dimensional culture in hydrogels accelerates retroviral gene transduction of human mesenchymal stem cells
AU - Choi, Bogyu
AU - Lee, Yein
AU - Han, Dong Keun
AU - Lee, Soo Hong
N1 - Publisher Copyright:
© 2019 Omnipress - All rights reserved.
PY - 2019
Y1 - 2019
N2 - Statement of Purpose: Numerous studies demonstrated that environmental dimensionality plays an important role in cell behavior.1,2 Cells cultured in three-dimensional (3D) environments markedly differ from those cultured on conventional two-dimensional (2D) tissue culture plates. There are numerous approaches to improve the low transduction efficiency of retroviral vectors.4-6 However, most studies investigating whether cell cycle regulation enhances retroviral gene transduction have been performed in 2D environments, and the effect of a 3D microenvironment, which better mimics in vivo conditions, is unknown. Cytocompatible hyaluronic acid (HA) hydrogels are a good candidate to study this issue. In this study, we are reporting a novel priming method for efficient and rapid retroviral gene transduction using photocrosslinkable HA hydrogels.
AB - Statement of Purpose: Numerous studies demonstrated that environmental dimensionality plays an important role in cell behavior.1,2 Cells cultured in three-dimensional (3D) environments markedly differ from those cultured on conventional two-dimensional (2D) tissue culture plates. There are numerous approaches to improve the low transduction efficiency of retroviral vectors.4-6 However, most studies investigating whether cell cycle regulation enhances retroviral gene transduction have been performed in 2D environments, and the effect of a 3D microenvironment, which better mimics in vivo conditions, is unknown. Cytocompatible hyaluronic acid (HA) hydrogels are a good candidate to study this issue. In this study, we are reporting a novel priming method for efficient and rapid retroviral gene transduction using photocrosslinkable HA hydrogels.
UR - http://www.scopus.com/inward/record.url?scp=85065428303&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85065428303
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
SP - 685
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -