Priming via three-dimensional culture in hydrogels accelerates retroviral gene transduction of human mesenchymal stem cells

Bogyu Choi, Yein Lee, Dong Keun Han, Soo Hong Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Statement of Purpose: Numerous studies demonstrated that environmental dimensionality plays an important role in cell behavior.1,2 Cells cultured in three-dimensional (3D) environments markedly differ from those cultured on conventional two-dimensional (2D) tissue culture plates. There are numerous approaches to improve the low transduction efficiency of retroviral vectors.4-6 However, most studies investigating whether cell cycle regulation enhances retroviral gene transduction have been performed in 2D environments, and the effect of a 3D microenvironment, which better mimics in vivo conditions, is unknown. Cytocompatible hyaluronic acid (HA) hydrogels are a good candidate to study this issue. In this study, we are reporting a novel priming method for efficient and rapid retroviral gene transduction using photocrosslinkable HA hydrogels.

Original languageEnglish
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Pages685
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: 3 Apr 20196 Apr 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Country/TerritoryUnited States
CitySeattle
Period3/04/196/04/19

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