Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

Jung Ho Ko, Chul Sang Lee, Kui Hyun Kim, Myung Geol Pang, Ja Shin Koo, Nanzhu Fang, Deog Bon Koo, Keon Bong Oh, Woo Sik Youn, Guo Dong Zheng, Jung Sun Park, Sun Jung Kim, Yong Mahn Han, In Young Choi, Joonho Lim, Sang Tae Shin, Seung Won Jin, Kyung Kwang Lee, Ook Joon Yoo

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalTransgenic Research
Volume9
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Biological activity
  • Bioreactor
  • G-CSF
  • O-glycosylation
  • Transgenic goat

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