Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

Jung Ho Ko; Chul Sang Lee; Kui Hyun Kim; Myung Geol Pang; Ja Shin Koo; Nanzhu Fang; Deog Bon Koo; Keon Bong Oh; Woo Sik Youn; Guo Dong Zheng; Jung Sun Park; Sun Jung Kim; Yong Mahn Han; In Young Choi; Joonho Lim; Sang Tae Shin; Seung Won Jin; Kyung Kwang Lee; Ook Joon Yoo

doi:10.1023/A:1008972010351

Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

Jung Ho Ko
, Chul Sang Lee
, Kui Hyun Kim
, Myung Geol Pang
, Ja Shin Koo
, Nanzhu Fang
, Deog Bon Koo
, Keon Bong Oh
, Woo Sik Youn
, Guo Dong Zheng
, Jung Sun Park
, Sun Jung Kim
, Yong Mahn Han
, In Young Choi
, Joonho Lim
, Sang Tae Shin
, Seung Won Jin
, Kyung Kwang Lee
, Ook Joon Yoo

Department of Life Science

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.

Original language	English
Pages (from-to)	215-222
Number of pages	8
Journal	Transgenic Research
Volume	9
Issue number	3
DOIs	https://doi.org/10.1023/A:1008972010351
State	Published - 2000

Keywords

Biological activity
Bioreactor
G-CSF
O-glycosylation
Transgenic goat

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10.1023/A:1008972010351

Cite this

Ho Ko, J., Lee, C. S., Kim, K. H., Pang, M. G., Koo, J. S., Fang, N., Koo, D. B., Oh, K. B., Youn, W. S., Zheng, G. D., Park, J. S., Kim, S. J., Han, Y. M., Choi, I. Y., Lim, J., Shin, S. T., Jin, S. W., Lee, K. K., & Yoo, O. J. (2000). Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat. Transgenic Research, 9(3), 215-222. https://doi.org/10.1023/A:1008972010351

@article{f427d349ca984c1aa78c103b21c08bac,

title = "Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat",

abstract = "We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.",

keywords = "Biological activity, Bioreactor, G-CSF, O-glycosylation, Transgenic goat",

author = "\{Ho Ko\}, Jung and Lee, \{Chul Sang\} and Kim, \{Kui Hyun\} and Pang, \{Myung Geol\} and Koo, \{Ja Shin\} and Nanzhu Fang and Koo, \{Deog Bon\} and Oh, \{Keon Bong\} and Youn, \{Woo Sik\} and Zheng, \{Guo Dong\} and Park, \{Jung Sun\} and Kim, \{Sun Jung\} and Han, \{Yong Mahn\} and Choi, \{In Young\} and Joonho Lim and Shin, \{Sang Tae\} and Jin, \{Seung Won\} and Lee, \{Kyung Kwang\} and Yoo, \{Ook Joon\}",

year = "2000",

doi = "10.1023/A:1008972010351",

language = "English",

volume = "9",

pages = "215--222",

journal = "Transgenic Research",

issn = "0962-8819",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "3",

}

Ho Ko, J, Lee, CS, Kim, KH, Pang, MG, Koo, JS, Fang, N, Koo, DB, Oh, KB, Youn, WS, Zheng, GD, Park, JS, Kim, SJ, Han, YM, Choi, IY, Lim, J, Shin, ST, Jin, SW, Lee, KK & Yoo, OJ 2000, 'Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat', Transgenic Research, vol. 9, no. 3, pp. 215-222. https://doi.org/10.1023/A:1008972010351

TY - JOUR

T1 - Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

AU - Ho Ko, Jung

AU - Lee, Chul Sang

AU - Kim, Kui Hyun

AU - Pang, Myung Geol

AU - Koo, Ja Shin

AU - Fang, Nanzhu

AU - Koo, Deog Bon

AU - Oh, Keon Bong

AU - Youn, Woo Sik

AU - Zheng, Guo Dong

AU - Park, Jung Sun

AU - Kim, Sun Jung

AU - Han, Yong Mahn

AU - Choi, In Young

AU - Lim, Joonho

AU - Shin, Sang Tae

AU - Jin, Seung Won

AU - Lee, Kyung Kwang

AU - Yoo, Ook Joon

PY - 2000

Y1 - 2000

N2 - We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.

AB - We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 μg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.

KW - Biological activity

KW - Bioreactor

KW - G-CSF

KW - O-glycosylation

KW - Transgenic goat

UR - https://www.scopus.com/pages/publications/0033800863

U2 - 10.1023/A:1008972010351

DO - 10.1023/A:1008972010351

M3 - Article

C2 - 11032370

AN - SCOPUS:0033800863

SN - 0962-8819

VL - 9

SP - 215

EP - 222

JO - Transgenic Research

JF - Transgenic Research

IS - 3

ER -

Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

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Keywords

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