Protective effects of myricetin on benzo[a]pyrene-induced 8-hydroxy-2-deoxyguanosine and BPDE-DNA adduct

Seung Cheol Jee, Min Kim, Kyeong Seok Kim, Hyung Sik Kim, Jung Suk Sung

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Benzo[a]pyrene (B[a]P), a group 1 carcinogen, induces mutagenic DNA adducts. Myricetin is present in many natural foods with diverse biological activities, such as anti-oxidative and anti-cancer activities. The aim of this study was to investigate the protective effects of myricetin against B[a]P-induced toxicity. Treatment of B[a]P induced cytotoxicity on HepG2 cells, whereas co-treatment of myricetin with B[a]P reduced the formation of the B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adduct, which recovered cell viability. Furthermore, we found a protective effect of myricetin against B[a]P-induced genotoxicity in rats, via myricetin-induced inhibition of 8-hydroxy-2-deoxyguanosine (8-OHdG) and BPDE-DNA adduct formation in the liver, kidney, colon, and stomach tissue. This inhibition was more prominent in the liver than in other tissues. Correspondingly, myricetin regulated the phase I and II enzymes that inhibit B[a]P metabolism and B[a]P metabolites conjugated with DNA by reducing and inducing CYP1A1 and glutathione S-transferase (GST) expression, respectively. Taken together, this showed that myricetin attenuated B[a]P-induced genotoxicity via regulation of phase I and II enzymes. Our results suggest that myricetin is anti-genotoxic, and prevents oxidative DNA damage and BPDE-DNA adduct formation via regulation of phase I and II enzymes.

Original languageEnglish
Article number446
JournalAntioxidants
Volume9
Issue number5
DOIs
StatePublished - May 2020

Keywords

  • Benzo[a]pyrene
  • BPDE-DNA adduct
  • Myricetin
  • Oxidative stress
  • Phase detoxifying enzyme

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