Quantification of lectin-exosome binding affinity using lectin-conjugated janus nano particles for detection of pancreatic cancer exosome

Statement of Purpose: In order to overcome difficulties in diagnosis of pancreatic cancer, various strategies have been extensively investigated. Exosomes (i.e., membrane vesicles of original cells ranging in size from 30 to 150 nm) could be utilized as a novel biomarker for cancer detection due to its endougenous components inside. Additionally, there is a higher frequency of polysaccharide presentation in exosome surfaces. Thus, lectin-mediated detection could be a sustitute of convnetional antibody-based cancer exosome detection assays. Therefore, for finding the optimal lectin, we have optimized quantification of binding affinity between lectin and exosome. Here, we developed (1) lectin-exosome binding assay for quantification of binding affinity of lectin and surface glycans expressed in pancreatic cancer exosome, and (2) lectin-conjugated Janus gold nanoparticles (JNPs), which has a potential that not only can capture exosomes via lectins conjugated onto polystyrene side, but also be used for further separation and analysis by the opposite gold side. Thus, we speculated that it is possible to determine optimal lectin through the quantified binding affinity and effectively capture cancer cell-derived exosomes in liquid biopsy by utilizing JNPs for efficient detection of pancreatic cancer exosome.