R5 Peptide-based Biosilicification Using Methyltrimethoxysilane

Jeong Chan Park; Do Hyeon Kim; Chang Sup Kim; Jeong Hyun Seo

doi:10.1007/s12257-017-0451-2

R5 Peptide-based Biosilicification Using Methyltrimethoxysilane

Jeong Chan Park, Do Hyeon Kim, Chang Sup Kim, Jeong Hyun Seo

Department of Chemical and Biochemical Engineering

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.

Original language	English
Pages (from-to)	11-15
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	23
Issue number	1
DOIs	https://doi.org/10.1007/s12257-017-0451-2
State	Published - 1 Feb 2018

Keywords

biosilica
GFP-R5 fusion system
hydrophobic silane
methyltrimethoxysilane

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10.1007/s12257-017-0451-2

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title = "R5 Peptide-based Biosilicification Using Methyltrimethoxysilane",

abstract = "We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.",

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AU - Seo, Jeong Hyun

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AB - We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.

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R5 Peptide-based Biosilicification Using Methyltrimethoxysilane

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Keywords

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