Reinekea thalattae sp. nov., a New Species of the Genus Reinekea Isolated from Surface Seawater in Sehwa Beach

A Gram-stain-negative, non-pigmented, curved rod-shaped, single polarly flagellated, facultatively anaerobic bacterium, designated as SSH23^T, was isolated from surface seawater sample collected at the Sehwa Beach in South Korea. The novel isolate required NaCl for growth and grew optimally between 2 and 3% NaCl. Strain SSH23^T showed high 16S rRNA gene sequence similarities with Reinekea marinisedimentorum DSM 15388^T (96.4%), Reinekea marina KACC 17315^T (96.2%), Reinekea blandensis KACC 17315^T (95.9%) and Reinekea aestuarii KCTC 22813^T (95.6%). The major polar lipids of strain SSH23^T were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids of strain SSH23^T were C_16:0, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). The predominant respiratory quinone was found to be ubiquinone-8. The average nucleotide identity values of strain SSH23^T with R. marinisedimentorum DSM 15388^T and R. blandensis MED297^T were determined to be 72.2% and 69.8%, respectively. The G+C content of the genomic DNA was 45.5 mol%. Based on genotypic, phenotypic, chemotaxonomic, and phylogenetic analyses, strain SSH23^T was considered to represent a novel member of the genus Reinekea, for which the name Reinekea thalattae sp. nov. is proposed. The type strain of Reinekea thalattae is SSH23^T (= KACC 21168^T = NBRC 113795^T).