TY - JOUR
T1 - Rhizobium setariae sp. nov., an Indole-3-Acetic Acid-Producing Bacterium Isolated from Green Foxtail, Setaria viridis
AU - Kang, Minchung
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/6
Y1 - 2022/6
N2 - A Gram-negative, indole-3-acetic acid-producing, aerobic, motile strain, designated as KVB221T, was isolated from a green foxtail plant, Setaria viridis, from a park near the coast of Haeundae Beach, Busan, Republic of Korea. The 16S rRNA gene analysis revealed strain KVB221T to be a member of the genus Rhizobium, from which Rhizobium alvei TNR-22T (97.2%), Rhizobium daejeonense L61T (96.9%), and Rhizobium ipomoeae shin9-1T (95.7%) were selected for comparative analysis. Growth of the strain was observed at 10–50 °C (optimum 25–30 °C), at pH 5–10 (optimum pH 7), and in the presence of 0–8% NaCl (optimum 0%). The strain was observed to produce 36.3 ± 0.8 μg/ml of indole following 5 days of incubation. The major fatty acids are comprised of C16:0, C19:0 cyclo ω8c, C18:1ω7c, and the unresolved group summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), while major polar lipids are identified as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylmonomethylethanolamine (PME). The predominant quinone is Q-10 and the DNA G+C content of the strain is 59.3%. Based on publicly available genome data between strain KVB221T and its closely related strains, the average nucleotide identity and in silico DNA–DNA hybridization values ranged from 72.7 to 73.1 and 19.7 to 20.4%, respectively. Based on the chemotaxonomic, phenotypic, and genomic comparisons reported here, we propose Rhizobium setariae sp. nov. as a novel species belonging to the genus Rhizobium. The type strain is KVB221T (= KACC 21713T = NBRC 114644T).
AB - A Gram-negative, indole-3-acetic acid-producing, aerobic, motile strain, designated as KVB221T, was isolated from a green foxtail plant, Setaria viridis, from a park near the coast of Haeundae Beach, Busan, Republic of Korea. The 16S rRNA gene analysis revealed strain KVB221T to be a member of the genus Rhizobium, from which Rhizobium alvei TNR-22T (97.2%), Rhizobium daejeonense L61T (96.9%), and Rhizobium ipomoeae shin9-1T (95.7%) were selected for comparative analysis. Growth of the strain was observed at 10–50 °C (optimum 25–30 °C), at pH 5–10 (optimum pH 7), and in the presence of 0–8% NaCl (optimum 0%). The strain was observed to produce 36.3 ± 0.8 μg/ml of indole following 5 days of incubation. The major fatty acids are comprised of C16:0, C19:0 cyclo ω8c, C18:1ω7c, and the unresolved group summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), while major polar lipids are identified as phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and phosphatidylmonomethylethanolamine (PME). The predominant quinone is Q-10 and the DNA G+C content of the strain is 59.3%. Based on publicly available genome data between strain KVB221T and its closely related strains, the average nucleotide identity and in silico DNA–DNA hybridization values ranged from 72.7 to 73.1 and 19.7 to 20.4%, respectively. Based on the chemotaxonomic, phenotypic, and genomic comparisons reported here, we propose Rhizobium setariae sp. nov. as a novel species belonging to the genus Rhizobium. The type strain is KVB221T (= KACC 21713T = NBRC 114644T).
UR - http://www.scopus.com/inward/record.url?scp=85128417380&partnerID=8YFLogxK
U2 - 10.1007/s00284-022-02860-2
DO - 10.1007/s00284-022-02860-2
M3 - Article
C2 - 35435479
AN - SCOPUS:85128417380
SN - 0343-8651
VL - 79
JO - Current Microbiology
JF - Current Microbiology
IS - 6
M1 - 162
ER -