TY - JOUR
T1 - Rhodamine-azobenzene based single molecular probe for multiple ions sensing
T2 - Cu 2+ , Al 3+ , Cr 3+ and its imaging in human lymphocyte cells
AU - Mabhai, Subhabrata
AU - Dolai, Malay
AU - Dey, Surya Kanta
AU - Dhara, Anamika
AU - Choudhury, Sujata Maiti
AU - Das, Bhriguram
AU - Dey, Satyajit
AU - Jana, Atanu
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/8/5
Y1 - 2019/8/5
N2 - A photoinduced electron transfer (PET) and chelation-enhanced fluorescence (CHEF) regulated rhodamine-azobenzene chemosensor (L) was synthesized for chemoselective detection of Al 3+ , Cr 3+ , and Cu 2+ by UV–Visible absorption study whereas Al 3+ and Cr 3+ by fluorimetric study in EtOH-H 2 O solvent. L showed a clear fluorescence emission enhancement of 21 and 16 fold upon addition of Al 3+ and Cr 3+ due to the 1:1 host-guest complexation, respectively. This is first report on rhodamine-azobenzene based Cr 3+ chemosensor. The complex formation, restricted imine isomerization, inhibition of PET (photo-induced electron transfer) process with the concomitant opening of the spirolactam ring induced a turn-on fluorescence response. The higher binding constants 6.7 × 10 3 M −1 and 3.8 × 10 3 M −1 for Al 3+ and Cr 3+ , respectively and lower detection limits 1 × 10 −6 M and 2 × 10 −6 M for Al 3+ and Cr 3+ , respectively in a buffered solution with high reversible nature describes the potential of L as an effective tool for detecting Al 3+ and Cr 3+ in a biological system with higher intracellular resolution. Finally, L was used to map the intracellular concentration of Al 3+ and Cr 3+ in human lymphocyte cells (HLCs) at physiological pH very effectively. Altogether, our findings will pave the way for designing new chemosensors for multiple analytes and those chemosensors will be effective for cell imaging study.
AB - A photoinduced electron transfer (PET) and chelation-enhanced fluorescence (CHEF) regulated rhodamine-azobenzene chemosensor (L) was synthesized for chemoselective detection of Al 3+ , Cr 3+ , and Cu 2+ by UV–Visible absorption study whereas Al 3+ and Cr 3+ by fluorimetric study in EtOH-H 2 O solvent. L showed a clear fluorescence emission enhancement of 21 and 16 fold upon addition of Al 3+ and Cr 3+ due to the 1:1 host-guest complexation, respectively. This is first report on rhodamine-azobenzene based Cr 3+ chemosensor. The complex formation, restricted imine isomerization, inhibition of PET (photo-induced electron transfer) process with the concomitant opening of the spirolactam ring induced a turn-on fluorescence response. The higher binding constants 6.7 × 10 3 M −1 and 3.8 × 10 3 M −1 for Al 3+ and Cr 3+ , respectively and lower detection limits 1 × 10 −6 M and 2 × 10 −6 M for Al 3+ and Cr 3+ , respectively in a buffered solution with high reversible nature describes the potential of L as an effective tool for detecting Al 3+ and Cr 3+ in a biological system with higher intracellular resolution. Finally, L was used to map the intracellular concentration of Al 3+ and Cr 3+ in human lymphocyte cells (HLCs) at physiological pH very effectively. Altogether, our findings will pave the way for designing new chemosensors for multiple analytes and those chemosensors will be effective for cell imaging study.
KW - Azobenzene
KW - CHEF
KW - Chemosensor
KW - PET
KW - Rhodamine
UR - http://www.scopus.com/inward/record.url?scp=85064891602&partnerID=8YFLogxK
U2 - 10.1016/j.saa.2019.04.056
DO - 10.1016/j.saa.2019.04.056
M3 - Article
C2 - 31054496
AN - SCOPUS:85064891602
SN - 1386-1425
VL - 219
SP - 319
EP - 332
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
ER -