TY - JOUR
T1 - ROS-mediated cytotoxic activity of ZnO and CeO2 nanoparticles synthesized using the Rubia cordifolia L. leaf extract on MG-63 human osteosarcoma cell lines
AU - Sisubalan, Natarajan
AU - Ramkumar, Vijayan Sri
AU - Pugazhendhi, Arivalagan
AU - Karthikeyan, Chandrasekaran
AU - Indira, Karuppusamy
AU - Gopinath, Kasi
AU - Hameed, Abdulrahman Syedahamed Haja
AU - Basha, Mohamed Hussain Ghouse
N1 - Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - In the present scenario, the synthesis and characterization of zinc oxide (ZnO) and cerium oxide (CeO2) nanoparticles (NPs) through biological routes using green reducing agents are quite interesting to explore various biomedical and pharmaceutical applications, particularly for the treatment of cancer. This study was focused on the phytosynthesis of ZnO and CeO2 NPs using the leaf extract of Rubia cordifolia L. The active principles present in the plant extract were liable for rapid reduction of Zn and Ce ions to metallic nanocrystals. ZnO and CeO2 NPs were characterized by UV–visible spectroscopy, X-ray diffraction analysis (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDAX), and photoluminescence (PL) techniques. ZnO and CeO2 NPs were partially agglomerated with a net-like structure. Biomedical activities of ZnO and CeO2 NPs were tested against MG-63 human osteosarcoma cells using MTT and reactive oxygen species (ROS) quantification assays. In treated cells, loss of cell membrane integrity, oxidative stress, and apoptosis was observed and it is well correlated with cellular damage immediately after induction. Overall, this study shed light on the anti-cancer potential of ZnO and CeO2 NPs on MG-63 human osteosarcoma cells through differential ROS production pathways, describing the potential role of greener synthesis.
AB - In the present scenario, the synthesis and characterization of zinc oxide (ZnO) and cerium oxide (CeO2) nanoparticles (NPs) through biological routes using green reducing agents are quite interesting to explore various biomedical and pharmaceutical applications, particularly for the treatment of cancer. This study was focused on the phytosynthesis of ZnO and CeO2 NPs using the leaf extract of Rubia cordifolia L. The active principles present in the plant extract were liable for rapid reduction of Zn and Ce ions to metallic nanocrystals. ZnO and CeO2 NPs were characterized by UV–visible spectroscopy, X-ray diffraction analysis (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDAX), and photoluminescence (PL) techniques. ZnO and CeO2 NPs were partially agglomerated with a net-like structure. Biomedical activities of ZnO and CeO2 NPs were tested against MG-63 human osteosarcoma cells using MTT and reactive oxygen species (ROS) quantification assays. In treated cells, loss of cell membrane integrity, oxidative stress, and apoptosis was observed and it is well correlated with cellular damage immediately after induction. Overall, this study shed light on the anti-cancer potential of ZnO and CeO2 NPs on MG-63 human osteosarcoma cells through differential ROS production pathways, describing the potential role of greener synthesis.
KW - Anti-carcinomal activity
KW - CeO
KW - Green synthesis
KW - Rubia cordifolia L
KW - SEM
KW - ZnO
UR - http://www.scopus.com/inward/record.url?scp=85030170250&partnerID=8YFLogxK
U2 - 10.1007/s11356-017-0003-5
DO - 10.1007/s11356-017-0003-5
M3 - Article
C2 - 28963600
AN - SCOPUS:85030170250
SN - 0944-1344
VL - 25
SP - 10482
EP - 10492
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 11
ER -