TY - JOUR
T1 - Roseateles albus sp. nov., Roseateles koreensis sp. nov. and Janthinobacterium fluminis sp. nov., isolated from freshwater at Jucheon River, and emended description of Roseateles aquaticus comb. nov.
AU - Park, Sunho
AU - Kim, Inhyup
AU - Chhetri, Geeta
AU - So, Yoonseop
AU - Jung, Yonghee
AU - Woo, Haejin
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2023 The Authors.
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Three Gram-stain-negative, facultatively anaerobic, rod-shaped, catalase-positive, oxidase-negative bacterial strains were designated as hw1T, hw8T and hw3T. Strains hw1T, hw8T and hw3T grew at 15–28°C (optimum, 25°C), 15–35°C (optimum, 30°C) and 4–28°C (optimum, 20°C), respectively, and at pH 7.0–12.0 (optimum, pH 9.0), pH 6.0–11.0 (optimum, pH 9.0) and 5.0–12.0 (optimum, pH 7.0), respectively. Additionally, strains hw1T and hw8T only grew when the NaCl concentration was 0%, while strain hw3T grew at between 0 and 0.5% (w/v; optimum, 0%). The average nucleotide identity (ANI) values between strains hw1T, hw8T and the Roseateles type strains ranged from 73.8 to 84.2%, while the digital DNA–DNA hybridization (dDDH) values ranged from 19.7 to 27.5%. The ANI values between strain hw3T and the Janthinobacterium type strains ranged from 78.7 to 80.7%, while dDDH values ranged from 22.3 to 23.0%. The draft genomes of strains hw1T, hw8T and hw3T consisted of 5.5, 4.4 and 5.9 Mbp, with DNA G+C contents of 61.7, 61.8 and 66.0mol%, respectively. The results of the dDDH, ANI, phylogenetic, biochemical and physiological analyses indicated that the novel strains were distinct from other members of their genera. Thus, we proposed the names Roseateles albus sp. nov. (type strain hw1T= KACC 22887T= TBRC 16613T), Roseateles koreensis sp. nov. (type strain hw8T= KACC 22885T= TBRC 16614T) and Janthinobacterium fluminis sp. nov. (type strain hw3T= KACC 22886T= TBRC 16615T).
AB - Three Gram-stain-negative, facultatively anaerobic, rod-shaped, catalase-positive, oxidase-negative bacterial strains were designated as hw1T, hw8T and hw3T. Strains hw1T, hw8T and hw3T grew at 15–28°C (optimum, 25°C), 15–35°C (optimum, 30°C) and 4–28°C (optimum, 20°C), respectively, and at pH 7.0–12.0 (optimum, pH 9.0), pH 6.0–11.0 (optimum, pH 9.0) and 5.0–12.0 (optimum, pH 7.0), respectively. Additionally, strains hw1T and hw8T only grew when the NaCl concentration was 0%, while strain hw3T grew at between 0 and 0.5% (w/v; optimum, 0%). The average nucleotide identity (ANI) values between strains hw1T, hw8T and the Roseateles type strains ranged from 73.8 to 84.2%, while the digital DNA–DNA hybridization (dDDH) values ranged from 19.7 to 27.5%. The ANI values between strain hw3T and the Janthinobacterium type strains ranged from 78.7 to 80.7%, while dDDH values ranged from 22.3 to 23.0%. The draft genomes of strains hw1T, hw8T and hw3T consisted of 5.5, 4.4 and 5.9 Mbp, with DNA G+C contents of 61.7, 61.8 and 66.0mol%, respectively. The results of the dDDH, ANI, phylogenetic, biochemical and physiological analyses indicated that the novel strains were distinct from other members of their genera. Thus, we proposed the names Roseateles albus sp. nov. (type strain hw1T= KACC 22887T= TBRC 16613T), Roseateles koreensis sp. nov. (type strain hw8T= KACC 22885T= TBRC 16614T) and Janthinobacterium fluminis sp. nov. (type strain hw3T= KACC 22886T= TBRC 16615T).
KW - average nucleotide identity
KW - digital DNA–DNA hybridization
KW - draft genome
KW - freshwater
KW - Janthinobacterium
KW - Roseateles
UR - http://www.scopus.com/inward/record.url?scp=85172279282&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.006043
DO - 10.1099/ijsem.0.006043
M3 - Article
C2 - 37750754
AN - SCOPUS:85172279282
SN - 1466-5026
VL - 73
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 9
M1 - 006043
ER -