Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing

Youngeun Kwon; Matthew A. Coleman; Julio A. Camarero

doi:10.1002/anie.200503475

Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing

Youngeun Kwon, Matthew A. Coleman, Julio A. Camarero

Department of Biomedical Engineering

Lawrence Livermore National Laboratory

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

(Figure Presented) Without a trace: The immobilization of biologically active proteins onto a solid support through split-intein-mediated protein trans-splicing reactions requires only very dilute samples. Not only is this process traceless but it does not require purification of the protein, thereby making possible the direct immobilization of proteins from complex mixtures such as cell lysates.

Original language	English
Pages (from-to)	1726-1729
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	11
DOIs	https://doi.org/10.1002/anie.200503475
State	Published - 3 Mar 2006

Keywords

Bioorganic chemistry
Biosensors
Immobilization
Protein engineering
Proteomics

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10.1002/anie.200503475

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abstract = "(Figure Presented) Without a trace: The immobilization of biologically active proteins onto a solid support through split-intein-mediated protein trans-splicing reactions requires only very dilute samples. Not only is this process traceless but it does not require purification of the protein, thereby making possible the direct immobilization of proteins from complex mixtures such as cell lysates.",

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AU - Camarero, Julio A.

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KW - Bioorganic chemistry

KW - Biosensors

KW - Immobilization

KW - Protein engineering

KW - Proteomics

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Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing

Abstract

Keywords

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