Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes

Yeo Jin Seo, Hee Chul Ahn, Eun Hae Lee, Jongchul Bang, Young Min Kang, Hee Eun Kim, Yeon Mi Lee, Kyungmin Kim, Byong Seok Choi, Joon Hwa Lee

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)2 duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)2 or d(CGTACG)2, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)2 in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)2≫d(CACGTG)2>d(CGTACG)2 through multiple sequence discrimination steps during the B-Z transition.

Original languageEnglish
Pages (from-to)4344-4350
Number of pages7
JournalFEBS Letters
Volume584
Issue number20
DOIs
StatePublished - 2010

Keywords

  • B-Z transition
  • Hydrogen exchange
  • NMR
  • Non-CG repeat DNA
  • Z-DNA
  • Z-DNA binding protein

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