Abstract
The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)2 duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)2 or d(CGTACG)2, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)2 in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)2≫d(CACGTG)2>d(CGTACG)2 through multiple sequence discrimination steps during the B-Z transition.
Original language | English |
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Pages (from-to) | 4344-4350 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 584 |
Issue number | 20 |
DOIs | |
State | Published - 2010 |
Keywords
- B-Z transition
- Hydrogen exchange
- NMR
- Non-CG repeat DNA
- Z-DNA
- Z-DNA binding protein