Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes

Yeo Jin Seo; Hee Chul Ahn; Eun Hae Lee; Jongchul Bang; Young Min Kang; Hee Eun Kim; Yeon Mi Lee; Kyungmin Kim; Byong Seok Choi; Joon Hwa Lee

doi:10.1016/j.febslet.2010.09.036

Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes

Yeo Jin Seo
, Hee Chul Ahn
, Eun Hae Lee
, Jongchul Bang
, Young Min Kang
, Hee Eun Kim
, Yeon Mi Lee
, Kyungmin Kim
, Byong Seok Choi
, Joon Hwa Lee

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The Zα domain of human ADAR1 (Zα_ADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Zα_ADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)₂ duplex similarly. We performed NMR experiments on complexes between the Zα_ADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)₂ or d(CGTACG)₂, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)₂ in the previous study suggests that Zα_ADAR1 exhibits the sequence preference of d(CGCGCG)₂≫d(CACGTG)₂>d(CGTACG)₂ through multiple sequence discrimination steps during the B-Z transition.

Original language	English
Pages (from-to)	4344-4350
Number of pages	7
Journal	FEBS Letters
Volume	584
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2010.09.036
State	Published - 2010

Keywords

B-Z transition
Hydrogen exchange
NMR
Non-CG repeat DNA
Z-DNA
Z-DNA binding protein

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10.1016/j.febslet.2010.09.036

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@article{7c4bcdde45a64385bb28018b8b76a581,

title = "Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes",

abstract = "The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)2 duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)2 or d(CGTACG)2, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)2 in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)2≫d(CACGTG)2>d(CGTACG)2 through multiple sequence discrimination steps during the B-Z transition.",

keywords = "B-Z transition, Hydrogen exchange, NMR, Non-CG repeat DNA, Z-DNA, Z-DNA binding protein",

author = "Seo, \{Yeo Jin\} and Ahn, \{Hee Chul\} and Lee, \{Eun Hae\} and Jongchul Bang and Kang, \{Young Min\} and Kim, \{Hee Eun\} and Lee, \{Yeon Mi\} and Kyungmin Kim and Choi, \{Byong Seok\} and Lee, \{Joon Hwa\}",

year = "2010",

doi = "10.1016/j.febslet.2010.09.036",

language = "English",

volume = "584",

pages = "4344--4350",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

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}

TY - JOUR

T1 - Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes

AU - Seo, Yeo Jin

AU - Ahn, Hee Chul

AU - Lee, Eun Hae

AU - Bang, Jongchul

AU - Kang, Young Min

AU - Kim, Hee Eun

AU - Lee, Yeon Mi

AU - Kim, Kyungmin

AU - Choi, Byong Seok

AU - Lee, Joon Hwa

PY - 2010

Y1 - 2010

N2 - The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)2 duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)2 or d(CGTACG)2, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)2 in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)2≫d(CACGTG)2>d(CGTACG)2 through multiple sequence discrimination steps during the B-Z transition.

AB - The Zα domain of human ADAR1 (ZαADAR1) preferentially binds Z-DNA rather than B-DNA with high binding affinity. ZαADAR1 binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)2 duplex similarly. We performed NMR experiments on complexes between the ZαADAR1 and non-CG-repeat DNA duplexes, d(CACGTG)2 or d(CGTACG)2, with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)2 in the previous study suggests that ZαADAR1 exhibits the sequence preference of d(CGCGCG)2≫d(CACGTG)2>d(CGTACG)2 through multiple sequence discrimination steps during the B-Z transition.

KW - B-Z transition

KW - Hydrogen exchange

KW - NMR

KW - Non-CG repeat DNA

KW - Z-DNA

KW - Z-DNA binding protein

UR - https://www.scopus.com/pages/publications/77957793667

U2 - 10.1016/j.febslet.2010.09.036

DO - 10.1016/j.febslet.2010.09.036

M3 - Article

C2 - 20875819

AN - SCOPUS:77957793667

SN - 0014-5793

VL - 584

SP - 4344

EP - 4350

JO - FEBS Letters

JF - FEBS Letters

IS - 20

ER -

Sequence discrimination of the Zα domain of human ADAR1 during B-Z transition of DNA duplexes

Abstract

Keywords

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