Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation

S. H. Park; B. M. Lee; M. G. Salas; M. Srivatanakul; R. H. Smith

doi:10.1007/s001220051575

Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation

S. H. Park, B. M. Lee, M. G. Salas, M. Srivatanakul, R. H. Smith

Department of Life Science

Texas A&M University

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The effect of additional virulence (vir) genes and size of plasmid T-DNA in Agrobacterium tumefaciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional virG, virGN54D, virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/virG plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species.

Original language	English
Pages (from-to)	1015-1020
Number of pages	6
Journal	Theoretical And Applied Genetics
Volume	101
Issue number	7
DOIs	https://doi.org/10.1007/s001220051575
State	Published - 2000

Keywords

Agrobacterium
Cotton
Rice
Tobacco
Virulence genes

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title = "Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation",

abstract = "The effect of additional virulence (vir) genes and size of plasmid T-DNA in Agrobacterium tumefaciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional virG, virGN54D, virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/virG plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species.",

keywords = "Agrobacterium, Cotton, Rice, Tobacco, Virulence genes",

author = "Park, {S. H.} and Lee, {B. M.} and Salas, {M. G.} and M. Srivatanakul and Smith, {R. H.}",

year = "2000",

doi = "10.1007/s001220051575",

language = "English",

volume = "101",

pages = "1015--1020",

journal = "Theoretical And Applied Genetics",

issn = "0040-5752",

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TY - JOUR

T1 - Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation

AU - Park, S. H.

AU - Lee, B. M.

AU - Salas, M. G.

AU - Srivatanakul, M.

AU - Smith, R. H.

PY - 2000

Y1 - 2000

N2 - The effect of additional virulence (vir) genes and size of plasmid T-DNA in Agrobacterium tumefaciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional virG, virGN54D, virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/virG plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species.

AB - The effect of additional virulence (vir) genes and size of plasmid T-DNA in Agrobacterium tumefaciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional virG, virGN54D, virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/virG plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species.

KW - Agrobacterium

KW - Cotton

KW - Rice

KW - Tobacco

KW - Virulence genes

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U2 - 10.1007/s001220051575

DO - 10.1007/s001220051575

M3 - Article

AN - SCOPUS:0033679085

SN - 0040-5752

VL - 101

SP - 1015

EP - 1020

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

IS - 7

ER -

Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation

Abstract

Keywords

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