Single-walled carbon nanotube biosensors using aptamers as molecular recognition elements

Hye Mi So, Keehoon Won, Yong Hwan Kim, Byoung Kye Kim, Beyong Hwan Ryu, Pil Sun Na, Hyojin Kim, Jeong O. Lee

Research output: Contribution to journalArticlepeer-review

517 Scopus citations

Abstract

We report the real-time detection of protein using SWNT-FET-based biosensors comprising DNA aptamers as molecular recognition elements. Anti-thrombin aptamers that are highly specific to serine protein thrombin were immobilized on the sidewall of a SWNT-FET using CDI-Tween linking molecules. The binding of thrombin aptamers to SWNT-FETs causes a rightward shift of the threshold gate voltages, presumably due to the negatively charged backbone of the DNA aptamers. While the addition of thrombin solution causes an abrupt decrease in the conductance of the thrombin aptamer immobilized SWNT-FET, no noticeable change was observed with elastase.

Original languageEnglish
Pages (from-to)11906-11907
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number34
DOIs
StatePublished - 31 Aug 2005

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