TY - JOUR
T1 - Species-specific variation of RPA-interacting protein (RIP) splice isoforms
AU - Kim, Kwangsoo
AU - Lee, Eun Ju
AU - Lee, Seung Hoon
AU - Seo, Taegun
AU - Jang, Ik Soon
AU - Park, Junsoo
AU - Lee, Je Ho
PY - 2009/1
Y1 - 2009/1
N2 - Replication Protein A (RPA) is a single stranded DNA-binding protein involved in DNA metabolic activities such as replication, repair, and recombination. RPA-Interacting Protein α (RIPα) was originally identified as a nuclear transporter of RPA in Xenopus. The human RIPα gene encodes several splice isoforms, of which hRIPα and hRIPβ are the major translation products in vivo. However, limited information is available about the alternative splicing of RIPα in eukaryotes, apart from that in humans. In this study, we examined the alternative splicing of RIPα in the Drosophila, Xenopus, and mouse system. We showed that the number of splice isoforms of RIPα was species-specific, and displayed a tendency to increase in higher eukaryotes. Moreover, a mouse ortholog of hRIPβ, mRIPβ2, was not SUMOylated, in contrast to hRIPβ. Based on these results, we suggest that the RIPα gene gains more splice isoforms and additional modifications after molecular evolution.
AB - Replication Protein A (RPA) is a single stranded DNA-binding protein involved in DNA metabolic activities such as replication, repair, and recombination. RPA-Interacting Protein α (RIPα) was originally identified as a nuclear transporter of RPA in Xenopus. The human RIPα gene encodes several splice isoforms, of which hRIPα and hRIPβ are the major translation products in vivo. However, limited information is available about the alternative splicing of RIPα in eukaryotes, apart from that in humans. In this study, we examined the alternative splicing of RIPα in the Drosophila, Xenopus, and mouse system. We showed that the number of splice isoforms of RIPα was species-specific, and displayed a tendency to increase in higher eukaryotes. Moreover, a mouse ortholog of hRIPβ, mRIPβ2, was not SUMOylated, in contrast to hRIPβ. Based on these results, we suggest that the RIPα gene gains more splice isoforms and additional modifications after molecular evolution.
KW - Alternative splicing
KW - Nuclear transport
KW - Replication protein A (RPA)
KW - RIPα
KW - RIPβ
KW - SUMOylation
UR - http://www.scopus.com/inward/record.url?scp=62349103889&partnerID=8YFLogxK
U2 - 10.5483/BMBRep.2009.42.1.022
DO - 10.5483/BMBRep.2009.42.1.022
M3 - Article
AN - SCOPUS:62349103889
SN - 1976-6696
VL - 42
SP - 22
EP - 27
JO - BMB Reports
JF - BMB Reports
IS - 1
ER -