Spheroid culture system methods and applications for mesenchymal stem cells

Na Eun Ryu, Soo Hong Lee, Hansoo Park

Research output: Contribution to journalReview articlepeer-review

342 Scopus citations

Abstract

Owing to the importance of stem cell culture systems in clinical applications, researchers have extensively studied them to optimize the culture conditions and increase efficiency of cell culture. A spheroid culture system provides a similar physicochemical environment in vivo by facilitating cell–cell and cell–matrix interaction to overcome the limitations of traditional monolayer cell culture. In suspension culture, aggregates of adjacent cells form a spheroid shape having wide utility in tumor and cancer research, therapeutic transplantation, drug screening, and clinical study, as well as organic culture. There are various spheroid culture methods such as hanging drop, gel embedding, magnetic levitation, and spinner culture. Lately, efforts are being made to apply the spheroid culture system to the study of drug delivery platforms and co-cultures, and to regulate differentiation and pluripotency. To study spheroid cell culture, various kinds of biomaterials are used as building forms of hydrogel, film, particle, and bead, depending upon the requirement. However, spheroid cell culture system has limitations such as hypoxia and necrosis in the spheroid core. In addition, studies should focus on methods to dissociate cells from spheroid into single cells.

Original languageEnglish
Article number1620
JournalCells
Volume8
Issue number12
DOIs
StatePublished - Dec 2019

Keywords

  • 3D cell culture
  • Biomaterials
  • Spheroid culture

Fingerprint

Dive into the research topics of 'Spheroid culture system methods and applications for mesenchymal stem cells'. Together they form a unique fingerprint.

Cite this