TY - JOUR
T1 - Sphingomonas edaphi sp. nov., a novel species isolated from beach soil in the Republic of Korea
AU - Kim, Hyungdong
AU - Chhetri, Geeta
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - A yellow-coloured, Gram-negative, motile, strictly aerobic bacterial strain, designated strain DAC4T, was isolated from a soil sample collected at Ahnmok Beach (Busan, Republic of Korea). The cells of strain DAC4T were rod-shaped and the colonies that formed were round and convex. The results of phylogenetic analysis based on the 16S rRNA gene sequence of strain DAC4T revealed that the bacterium belongs to the genus Sphingomonas, family Sphingomonadaceae, and that it was most closely related to Sphingomonas jaspsi DSM 18422T (98.01%), Sphingomonas rhizophila KACC 19189T (97.76%), Sphingomonas mesophila KCTC 62179T(97.30%), Sphingomonas sedimincola KCTC 12629T (97.16%) and Sphingomonas oryziterrae KCTC 22476T (97.05%). The major respiratory quinone was Q-10, and the major cellular fatty acids were summed feature 8 (C18:1 ω7c) and summed feature 3 (C16:1 ω7c/C16:1 ω6c). The whole genome DNA G+C content of strain DAC4T was 62.16 mol%. Phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipids, phosphatidylglycerol, phosphatidylcholine, four undefined glycolipids and an undefined lipid were detected in strain DAC4T, and the strain had sym-homospermidine as a major polyamine. The in silico DNA–DNA hybridization and average nucleotide identity values between strain DAC4T and the closely related taxa S. jaspsi and S. mesophila were 75.5/23.5% and 73.5/18.5%, respectively. The fluorimetric DNA–DNA hybridization results showed that strain DAC4T and S. rhizophila, S. sediminicola and S. oryziterrae have 37.1, 35.2 and 32.2% DNA similarity, respectively. Based on phylogenetic, phenotypic and chemotaxonomic distinctiveness, strain DAC4T (=KCTC 62107T=JCM 32377T) is classified as a novel species of the genus Sphingomonas, for which the name Sphingomonas edaphi sp. nov. is proposed.
AB - A yellow-coloured, Gram-negative, motile, strictly aerobic bacterial strain, designated strain DAC4T, was isolated from a soil sample collected at Ahnmok Beach (Busan, Republic of Korea). The cells of strain DAC4T were rod-shaped and the colonies that formed were round and convex. The results of phylogenetic analysis based on the 16S rRNA gene sequence of strain DAC4T revealed that the bacterium belongs to the genus Sphingomonas, family Sphingomonadaceae, and that it was most closely related to Sphingomonas jaspsi DSM 18422T (98.01%), Sphingomonas rhizophila KACC 19189T (97.76%), Sphingomonas mesophila KCTC 62179T(97.30%), Sphingomonas sedimincola KCTC 12629T (97.16%) and Sphingomonas oryziterrae KCTC 22476T (97.05%). The major respiratory quinone was Q-10, and the major cellular fatty acids were summed feature 8 (C18:1 ω7c) and summed feature 3 (C16:1 ω7c/C16:1 ω6c). The whole genome DNA G+C content of strain DAC4T was 62.16 mol%. Phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipids, phosphatidylglycerol, phosphatidylcholine, four undefined glycolipids and an undefined lipid were detected in strain DAC4T, and the strain had sym-homospermidine as a major polyamine. The in silico DNA–DNA hybridization and average nucleotide identity values between strain DAC4T and the closely related taxa S. jaspsi and S. mesophila were 75.5/23.5% and 73.5/18.5%, respectively. The fluorimetric DNA–DNA hybridization results showed that strain DAC4T and S. rhizophila, S. sediminicola and S. oryziterrae have 37.1, 35.2 and 32.2% DNA similarity, respectively. Based on phylogenetic, phenotypic and chemotaxonomic distinctiveness, strain DAC4T (=KCTC 62107T=JCM 32377T) is classified as a novel species of the genus Sphingomonas, for which the name Sphingomonas edaphi sp. nov. is proposed.
KW - DNA-DNA relatednessx
KW - Polyphasic
KW - Sphingomonas
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85079020318&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003780
DO - 10.1099/ijsem.0.003780
M3 - Article
C2 - 31596192
AN - SCOPUS:85079020318
SN - 1466-5026
VL - 70
SP - 522
EP - 529
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 1
M1 - 003780
ER -