Sphingomonas sabuli sp. Nov., a carotenoid-producing bacterium isolated from beach sand

Minchung Kang, Geeta Chhetri, Jiyoun Kim, Inhyup Kim, Taegun Seo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A Gram-stain-negative, aerobic and non-motile bacterium, strain sand1-3T, was isolated from beach sand collected from Haeundae Beach located in Busan, Republic of Korea. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, Sphingomonas daechungensis CH15-11T (97.0%), Sphingomonas edaphi DAC4T (96.8%), Sphingomonas xanthus AE3T (96.5%) and Sphingomonas oryziterrae YC6722T (96.0%) were selected for comparing phenotypic and chemotaxonomic characteristics. Cells of strain sand1-3T grew at 7–50 °C (optimum, 30–35 °C), pH 5.0–8.0 (optimum, pH 7.0–8.0) and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Major polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and C18:1 2-OH. Moreover, the sole respiratory quinone and major polyamine were identified as ubiquinone-10 and homospermidine, respectively. The genomic DNA G+C content was 65.9 mol%. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values of strain sand1-3T and its reference strains with publicly available genomes were 17.9–18.9%, 72.0–75.3% and 63.3–76.5% respectively. Based on polyphasic evidence, we propose Sphingomonas sabuli sp. nov. as a novel species within the genus Sphingomonas. The type strain is sand1-3T (=KCTC 82358T=NBRC 114538T).

Original languageEnglish
Article number004896
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume71
Issue number7
DOIs
StatePublished - 2021

Keywords

  • Beach sand
  • Carotenoids
  • Phylogenetic analysis
  • Sphingomonadaceae
  • Sphingomonas

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