TY - JOUR
T1 - Sphingomonas sabuli sp. Nov., a carotenoid-producing bacterium isolated from beach sand
AU - Kang, Minchung
AU - Chhetri, Geeta
AU - Kim, Jiyoun
AU - Kim, Inhyup
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2021 The Authors.
PY - 2021
Y1 - 2021
N2 - A Gram-stain-negative, aerobic and non-motile bacterium, strain sand1-3T, was isolated from beach sand collected from Haeundae Beach located in Busan, Republic of Korea. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, Sphingomonas daechungensis CH15-11T (97.0%), Sphingomonas edaphi DAC4T (96.8%), Sphingomonas xanthus AE3T (96.5%) and Sphingomonas oryziterrae YC6722T (96.0%) were selected for comparing phenotypic and chemotaxonomic characteristics. Cells of strain sand1-3T grew at 7–50 °C (optimum, 30–35 °C), pH 5.0–8.0 (optimum, pH 7.0–8.0) and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Major polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and C18:1 2-OH. Moreover, the sole respiratory quinone and major polyamine were identified as ubiquinone-10 and homospermidine, respectively. The genomic DNA G+C content was 65.9 mol%. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values of strain sand1-3T and its reference strains with publicly available genomes were 17.9–18.9%, 72.0–75.3% and 63.3–76.5% respectively. Based on polyphasic evidence, we propose Sphingomonas sabuli sp. nov. as a novel species within the genus Sphingomonas. The type strain is sand1-3T (=KCTC 82358T=NBRC 114538T).
AB - A Gram-stain-negative, aerobic and non-motile bacterium, strain sand1-3T, was isolated from beach sand collected from Haeundae Beach located in Busan, Republic of Korea. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, Sphingomonas daechungensis CH15-11T (97.0%), Sphingomonas edaphi DAC4T (96.8%), Sphingomonas xanthus AE3T (96.5%) and Sphingomonas oryziterrae YC6722T (96.0%) were selected for comparing phenotypic and chemotaxonomic characteristics. Cells of strain sand1-3T grew at 7–50 °C (optimum, 30–35 °C), pH 5.0–8.0 (optimum, pH 7.0–8.0) and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Major polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) and C18:1 2-OH. Moreover, the sole respiratory quinone and major polyamine were identified as ubiquinone-10 and homospermidine, respectively. The genomic DNA G+C content was 65.9 mol%. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values of strain sand1-3T and its reference strains with publicly available genomes were 17.9–18.9%, 72.0–75.3% and 63.3–76.5% respectively. Based on polyphasic evidence, we propose Sphingomonas sabuli sp. nov. as a novel species within the genus Sphingomonas. The type strain is sand1-3T (=KCTC 82358T=NBRC 114538T).
KW - Beach sand
KW - Carotenoids
KW - Phylogenetic analysis
KW - Sphingomonadaceae
KW - Sphingomonas
UR - http://www.scopus.com/inward/record.url?scp=85111352123&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.004896
DO - 10.1099/ijsem.0.004896
M3 - Article
C2 - 34323678
AN - SCOPUS:85111352123
SN - 1466-5026
VL - 71
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 7
M1 - 004896
ER -