Abstract
Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting.
Original language | English |
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Pages (from-to) | 829-836 |
Number of pages | 8 |
Journal | Biotechnology Letters |
Volume | 33 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2011 |
Keywords
- Chondrocyte
- De-differentiation
- Redifferentiation
- Spinner-flask culture