Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes

Tae Jin Lee, Suk Ho Bhang, Wan Guen La, Hee Seok Yang, Jun Yeup Seong, Haeshin Lee, Gun Il Im, Soo Hong Lee, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting.

Original languageEnglish
Pages (from-to)829-836
Number of pages8
JournalBiotechnology Letters
Volume33
Issue number4
DOIs
StatePublished - Apr 2011

Keywords

  • Chondrocyte
  • De-differentiation
  • Redifferentiation
  • Spinner-flask culture

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