TY - JOUR
T1 - Spirosoma areae sp. nov., Isolated from Soil
AU - Jang, Jun Hyeong
AU - Cha, Seho
AU - Lee, Dongwook
AU - Srinivasan, Sathiyaraj
AU - Jheong, Weonhwa
AU - Hwang, Ha Sung
AU - Kim, Myung Kyum
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media, LLC.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - A yellow-colored Gram-negative strain, Arct 1-12T, was isolated from a soil sample collected in Seoul Women’s University, South Korea, and grown on R2A agar at 25 °C. Growth of strain Arct 1-12T was observed at a temperature range of 15–30 °C (optimal 25 °C), but not at 4 or 42 °C. The strain tolerated up to 1% NaCl (w/v) and displayed optimal growth in the absence of NaCl. Growth occurred at pH 6.0–9.0 (optimally at pH 7). According to the 16S rRNA gene sequence, the strain is moderately related to Spirosoma spitsbergense DSM 19989T (93.54%), S. endophyticum EX36T (93.25%), S. linguale LMG 10896T (92%), S. luteum DSM 19990T (93.16%), S. panaciterrae DSM 21099T (91.09%), S. oryzae RHs22T (90.37%), and S. rigui WPCB118T (91.54%). Chemotaxonomic analyses revealed that strain Arct 1-12T possesses MK-7 as the predominant menaquinone, a polar lipid profile consisting of phosphatidylethanolamine, an unknown aminolipid, an unknown aminophospholipid, and an unknown lipid, and iso–C15:0, C16:1ω5c and Summed Feature 3 (C16:1ω6c and/or C16:1ω7c) as the major fatty acids. The DNA G+C content is 52.3 mol %. Based on polyphasic evidence, strain Arct 1-12T (=JCM 31025 T = KCTC 42814T) is classified as the type strain of a novel Spirosoma species for which the name Spirosoma areae sp. nov. is proposed.
AB - A yellow-colored Gram-negative strain, Arct 1-12T, was isolated from a soil sample collected in Seoul Women’s University, South Korea, and grown on R2A agar at 25 °C. Growth of strain Arct 1-12T was observed at a temperature range of 15–30 °C (optimal 25 °C), but not at 4 or 42 °C. The strain tolerated up to 1% NaCl (w/v) and displayed optimal growth in the absence of NaCl. Growth occurred at pH 6.0–9.0 (optimally at pH 7). According to the 16S rRNA gene sequence, the strain is moderately related to Spirosoma spitsbergense DSM 19989T (93.54%), S. endophyticum EX36T (93.25%), S. linguale LMG 10896T (92%), S. luteum DSM 19990T (93.16%), S. panaciterrae DSM 21099T (91.09%), S. oryzae RHs22T (90.37%), and S. rigui WPCB118T (91.54%). Chemotaxonomic analyses revealed that strain Arct 1-12T possesses MK-7 as the predominant menaquinone, a polar lipid profile consisting of phosphatidylethanolamine, an unknown aminolipid, an unknown aminophospholipid, and an unknown lipid, and iso–C15:0, C16:1ω5c and Summed Feature 3 (C16:1ω6c and/or C16:1ω7c) as the major fatty acids. The DNA G+C content is 52.3 mol %. Based on polyphasic evidence, strain Arct 1-12T (=JCM 31025 T = KCTC 42814T) is classified as the type strain of a novel Spirosoma species for which the name Spirosoma areae sp. nov. is proposed.
UR - http://www.scopus.com/inward/record.url?scp=85023168690&partnerID=8YFLogxK
U2 - 10.1007/s00284-017-1297-8
DO - 10.1007/s00284-017-1297-8
M3 - Article
C2 - 28698912
AN - SCOPUS:85023168690
SN - 0343-8651
VL - 74
SP - 1148
EP - 1152
JO - Current Microbiology
JF - Current Microbiology
IS - 10
ER -