Structure and tissue distribution of a trinucleotide-repeat-containing gene (cag-3) expressed specifically in the mouse brain

Jin Woo Ji; Hye Lim Yang; Sun Jung Kim

doi:10.1016/s1016-8478(23)13237-7

Structure and tissue distribution of a trinucleotide-repeat-containing gene (cag-3) expressed specifically in the mouse brain

Jin Woo Ji, Hye Lim Yang, Sun Jung Kim

Department of Life Science

Dongguk University

Research output: Contribution to journal › Article › peer-review

Abstract

Using in silico approaches and RACE we cloned a full length trinucleotide (CAG) repeat-containing cDNA (cag-3). The cDNA is 2478 bp long and the deduced polypeptide consists of 140 amino acids of which 73 are glutamines. The genomic sequence spans approximately 79 kb on mouse chromosome 7 and the gene is composed of four exons. Standard and real-time PCR analyses of several mouse tissues showed that the gene is exclusively expressed in the brain and is not detected in embryonic stages. Within the brain, it is expressed throughout the forebrain region with predominant expression in the hypothalamus and olfactory bulb and very low levels in the mid- and hind-brain.

Original language	English
Pages (from-to)	348-353
Number of pages	6
Journal	Molecules and Cells
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/s1016-8478(23)13237-7
State	Published - 2005

Keywords

Mouse Brain
Nuclear Localization Signal
Poly (Q)
RACE
Real Time PCR
Trinucleotide Repeat Sequence

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10.1016/s1016-8478(23)13237-7

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title = "Structure and tissue distribution of a trinucleotide-repeat-containing gene (cag-3) expressed specifically in the mouse brain",

abstract = "Using in silico approaches and RACE we cloned a full length trinucleotide (CAG) repeat-containing cDNA (cag-3). The cDNA is 2478 bp long and the deduced polypeptide consists of 140 amino acids of which 73 are glutamines. The genomic sequence spans approximately 79 kb on mouse chromosome 7 and the gene is composed of four exons. Standard and real-time PCR analyses of several mouse tissues showed that the gene is exclusively expressed in the brain and is not detected in embryonic stages. Within the brain, it is expressed throughout the forebrain region with predominant expression in the hypothalamus and olfactory bulb and very low levels in the mid- and hind-brain.",

keywords = "Mouse Brain, Nuclear Localization Signal, Poly (Q), RACE, Real Time PCR, Trinucleotide Repeat Sequence",

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T1 - Structure and tissue distribution of a trinucleotide-repeat-containing gene (cag-3) expressed specifically in the mouse brain

AU - Ji, Jin Woo

AU - Yang, Hye Lim

AU - Kim, Sun Jung

PY - 2005

Y1 - 2005

N2 - Using in silico approaches and RACE we cloned a full length trinucleotide (CAG) repeat-containing cDNA (cag-3). The cDNA is 2478 bp long and the deduced polypeptide consists of 140 amino acids of which 73 are glutamines. The genomic sequence spans approximately 79 kb on mouse chromosome 7 and the gene is composed of four exons. Standard and real-time PCR analyses of several mouse tissues showed that the gene is exclusively expressed in the brain and is not detected in embryonic stages. Within the brain, it is expressed throughout the forebrain region with predominant expression in the hypothalamus and olfactory bulb and very low levels in the mid- and hind-brain.

AB - Using in silico approaches and RACE we cloned a full length trinucleotide (CAG) repeat-containing cDNA (cag-3). The cDNA is 2478 bp long and the deduced polypeptide consists of 140 amino acids of which 73 are glutamines. The genomic sequence spans approximately 79 kb on mouse chromosome 7 and the gene is composed of four exons. Standard and real-time PCR analyses of several mouse tissues showed that the gene is exclusively expressed in the brain and is not detected in embryonic stages. Within the brain, it is expressed throughout the forebrain region with predominant expression in the hypothalamus and olfactory bulb and very low levels in the mid- and hind-brain.

KW - Mouse Brain

KW - Nuclear Localization Signal

KW - Poly (Q)

KW - RACE

KW - Real Time PCR

KW - Trinucleotide Repeat Sequence

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DO - 10.1016/s1016-8478(23)13237-7

M3 - Article

C2 - 16404148

AN - SCOPUS:33644863948

SN - 1016-8478

VL - 20

SP - 348

EP - 353

JO - Molecules and Cells

JF - Molecules and Cells

IS - 3

ER -

Structure and tissue distribution of a trinucleotide-repeat-containing gene (cag-3) expressed specifically in the mouse brain

Abstract

Keywords

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