TY - JOUR
T1 - Study of mixed function oxidase system in Aspergillus ochraceus (NCIM 1146)
AU - Saratale, G. D.
AU - Humnabadkar, R. P.
AU - Govindwar, S. P.
PY - 2007/12
Y1 - 2007/12
N2 - Aspergillus ochraceus (NCIM-1146) has shown the ability to degrade cholesterol, camphor and naphthalene, when 96 h grown mycelium incubated in medium containing these organic compounds. Presence of higher level of electron transport components and biotransformation enzyme activity were observed in Aspergillus ochraceus, when grown in potato dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and shows inhibition in the presence of CO, indicating cytochrome P-450 mediated reactions. A significant increase in the levels of electron transport components and biotransformation enzyme activity were observed in presence of different inducers (viz. cholesterol, camphor, naphthalene, veratrole, phenobarbital, n-hexane, kerosene and saffola oil) when compared with mycelium incubated in same way with similar conditions for 2 min incubation. Analyses of the products of cholesterol and camphor using HPLC and GCMS confirm the degradation of these compounds.
AB - Aspergillus ochraceus (NCIM-1146) has shown the ability to degrade cholesterol, camphor and naphthalene, when 96 h grown mycelium incubated in medium containing these organic compounds. Presence of higher level of electron transport components and biotransformation enzyme activity were observed in Aspergillus ochraceus, when grown in potato dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and shows inhibition in the presence of CO, indicating cytochrome P-450 mediated reactions. A significant increase in the levels of electron transport components and biotransformation enzyme activity were observed in presence of different inducers (viz. cholesterol, camphor, naphthalene, veratrole, phenobarbital, n-hexane, kerosene and saffola oil) when compared with mycelium incubated in same way with similar conditions for 2 min incubation. Analyses of the products of cholesterol and camphor using HPLC and GCMS confirm the degradation of these compounds.
KW - Biotransformation enzymes
KW - Camphor
KW - Cholesterol
KW - Cytochrome c reductase
KW - Hexane oxidase
KW - N-hexane
KW - Naphthalene
UR - http://www.scopus.com/inward/record.url?scp=38149065946&partnerID=8YFLogxK
U2 - 10.1007/s12088-007-0056-0
DO - 10.1007/s12088-007-0056-0
M3 - Article
AN - SCOPUS:38149065946
SN - 0046-8991
VL - 47
SP - 304
EP - 309
JO - Indian Journal of Microbiology
JF - Indian Journal of Microbiology
IS - 4
ER -