Suppression of transglutaminase-2 is involved in anti-inflammatory actions of glucosamine in 12-O-Tetradecanoylphorbol- 13-acetate-induced skin inflammation

Mi Kyung Park, Sun A. Cho, Hye Ja Lee, Eun Ji Lee, June Hee Kang, You Lee Kim, Hyun Ji Kim, Seung Hyun Oh, Changsun Choi, Ho Lee, Soo Youl Kim, Chang Hoon Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Glucosamine (GS) is well known for the treatment of infl ammation. However, the mechanism and effi cacy of GS for skin infl ammation are unclear. The aim of this study was to evaluate the effects and mechanism of GS in the mouse 12-O-tetradecanoyl 13-acetate (TPA)-induced ear edema model. TPA-induced ear edema was evoked in ICR or transglutaminase 2 (Tgase-2) (-/-) mice. GS was administered orally (10-100 mg/kg) or topically (0.5-2.0 w/v %) prior to TPA treatment. Orally administered GS at 10 mg/kg showed a 76 or 57% reduction in ear weight or myeloperoxidase, respectively, and a decreased expression of cyclooxygenase- 2 (COX-2), NF-κB and Tgase-2 in TPA-induced ear edema by western blot and immunohistochemistry. Role of Tgase-2 in TPA ear edema is examined using Tgase-2 (-/-) mice and TPA did not induce COX-2 expression in ear of Tgase-2 (-/-) mice. These observations suggested that Tgase-2 is involved in TPA-induced COX-2 expression in the infl amed ear of mice and antiinfl ammatory effects of glucosamine is mediated through suppression of Tgase-2 in TPA ear edema.

Original languageEnglish
Pages (from-to)380-385
Number of pages6
JournalBiomolecules and Therapeutics
Volume20
Issue number4
DOIs
StatePublished - 2012

Keywords

  • Cyclooxygenase-2
  • Glucosamine
  • NF-κB
  • Tgase-2 (-/-) mice
  • TPA-induced ear edema
  • Transglutaminase-2

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