TY - JOUR
T1 - Surface functionalization of halloysite nanotubes with supermagnetic iron oxide, chitosan and 2-D calcium-phosphate nanoflakes for synergistic osteoconduction enhancement of human adipose tissue-derived mesenchymal stem cells
AU - Lee, Yoo Jung
AU - Lee, Seung Cheol
AU - Jee, Seung Cheol
AU - Sung, Jung Suk
AU - Kadam, Avinash A.
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Halloysite nanotubes (HNTs) are known to be the highly emerging materials in nano-medicinal applications. However, comprehensive exploitation of HNTs for the regenerative medicinal applications is still necessary to be done. Therefore, towards enhancing the osteogenic potential of human adipose tissue-derived mesenchymal stem cells (hADMSCs), this study synthesized a novel and multifunctional nanoscaffold of chitosan (CTs) functionalized supermagnetic halloysite nanotubes (M-HNTs) decorated with the calcium phosphate 2-D nanoflakes (CaP) (termed as; M-HNTs-CTs-CaP). Stepwise modified nanoscaffolds were characterized by FE-SEM, FE-SEM-EDS, FE-HR-TEM, XPS, FT-IR and VSM analyses. The hADMSCs osteogenic potential was confirmed by calcification (Alizarin Red S staining), phosphate quantification and immunocytochemistry. Nanoscaffolds; CaP, M-HNTs-CaP and M-HNTs-CTs-CaP were significantly enhanced and up-regulated osteogenic potential compared to the HNTs, M-HNTs, M-HNTs-CTs. Among the nanoscaffolds studied, M-HNTs-CTs-CaP exhibited highest osteogenesis, due to the enhanced CaP distribution on M-HNTs-CTs surface, and synergistic osteoconduction contributed from Fe 3 O 4 , chitosan and CaP. Moreover, immunocytochemistry analysis and morphologically observation showed well differentiated osteoblast on the M-HNTs-CTs-CaP surface. Therefore, M-HNTs-CTs-CaP found to have a strong osteogenic potential of hADMSCs, and might be serve as highly applicable, next generation nanoscaffold for bone tissue engineering application.
AB - Halloysite nanotubes (HNTs) are known to be the highly emerging materials in nano-medicinal applications. However, comprehensive exploitation of HNTs for the regenerative medicinal applications is still necessary to be done. Therefore, towards enhancing the osteogenic potential of human adipose tissue-derived mesenchymal stem cells (hADMSCs), this study synthesized a novel and multifunctional nanoscaffold of chitosan (CTs) functionalized supermagnetic halloysite nanotubes (M-HNTs) decorated with the calcium phosphate 2-D nanoflakes (CaP) (termed as; M-HNTs-CTs-CaP). Stepwise modified nanoscaffolds were characterized by FE-SEM, FE-SEM-EDS, FE-HR-TEM, XPS, FT-IR and VSM analyses. The hADMSCs osteogenic potential was confirmed by calcification (Alizarin Red S staining), phosphate quantification and immunocytochemistry. Nanoscaffolds; CaP, M-HNTs-CaP and M-HNTs-CTs-CaP were significantly enhanced and up-regulated osteogenic potential compared to the HNTs, M-HNTs, M-HNTs-CTs. Among the nanoscaffolds studied, M-HNTs-CTs-CaP exhibited highest osteogenesis, due to the enhanced CaP distribution on M-HNTs-CTs surface, and synergistic osteoconduction contributed from Fe 3 O 4 , chitosan and CaP. Moreover, immunocytochemistry analysis and morphologically observation showed well differentiated osteoblast on the M-HNTs-CTs-CaP surface. Therefore, M-HNTs-CTs-CaP found to have a strong osteogenic potential of hADMSCs, and might be serve as highly applicable, next generation nanoscaffold for bone tissue engineering application.
KW - Bone tissue engineering
KW - Calcium phosphate nanoflakes
KW - hADMSCs
KW - Halloysite nanotubes
KW - Multifunctional nanoscaffolds
KW - Osteoconduction
UR - http://www.scopus.com/inward/record.url?scp=85054337258&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.09.045
DO - 10.1016/j.colsurfb.2018.09.045
M3 - Article
C2 - 30261345
AN - SCOPUS:85054337258
SN - 0927-7765
VL - 173
SP - 18
EP - 26
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -