Tethering single DNA under electric field in a nanochannel

Byung Chul Lee, Chanmin Kang, Jinsik Kim, Ji Yoon Kang, Hyun Joon Shin, Sang Youp Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Current study introduces a simple DNA tethering method using electrodynamics in nanochannels. λ-DNAs were dielectrophoretically tethered in SiO2 nanochannels due to the particular cross-sectional geometry and DNA molecules showed tethering and stretching behaviors simultaneously at high electrical fields (above 20 kV/m). The tethering behavior was characterized by a commercial finite element method (FEM). Because the electric field gradient was very local at the nanohorn area, one end of DNA was able to be anchored by dielectrophoretic force, while electrostatic force of the same field elongates simultaneously the other part of the molecule.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages869-871
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Dielectrophoresis (DEP)
  • DNA manipulation
  • Nano-horn
  • Nanochannel

Fingerprint

Dive into the research topics of 'Tethering single DNA under electric field in a nanochannel'. Together they form a unique fingerprint.

Cite this