The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)

Nahui Kim; Juyeol Oh; Bichseam Kim; Eung Kyu Choi; Un Sun Hwang; Jack E. Staub; Sang Min Chung; Younghoon Park

doi:10.1007/s13580-015-0024-2

The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)

Nahui Kim, Juyeol Oh, Bichseam Kim, Eung Kyu Choi, Un Sun Hwang, Jack E. Staub, Sang Min Chung, Younghoon Park

Department of Life Science

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Most melon (Cucumis melo L.) breeding lines in South Korea display andromonoecious sex expression, which necessitates laborious hand emasculation during F₁ hybrid seed production. Thus, there is a need to develop monoecious sex types in elite germplasm to obviate self-pollination. Sex expression is associated with floral ethylene production, which, in monecious melon plants, is associated with the A locus. Our study was conducted to develop molecular markers for selection of monoecious plants based on sequence variation inherent in the CmACS-7 gene [encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)] that is associated with ethylene production. Full-length CmACS-7 sequences were cloned from a monoecious (MO23) and an andromonoecious (AM24) line. The alignment of those CmACS-7 sequences revealed a single nucleotide polymorphism (SNP; C170T) in exon 1 and an 18 bp indel in the 3′-untranslated region (UTR) of between MO23 and AM24, which was then used to develop a cleaved amplified polymorphic sequence (CAPS) (EX1-C170T) and a sequence characterized amplified region (SCAR) marker (T1ex), respectively. The sex expression and the T1ex SCAR-based genotype of 442 F₂ plants derived from a MO23 × AM24 cross was determined. Monoecy and andromonoecy segregated in a 3:1 ratio in F₂ progeny, where the sex type of 429 plants (13 plants not classified) co-segregated with the SCAR marker, demonstrating that sex expression regulated by CmACS-7 is controlled by a single dominant gene and that it confers monoecy in line MO23. Allelic variation in 112 geographically diverse melon lines for CmACS-7 as accessed by CAPS EX1-C170T and SCAR T1ex markers indicated that the: 1) exon 1 of CmACS-7 is highly conserved and the SNP/sex expression association detected is highly predictable making it potentially useful for marker-based selection of monoecious plants, and; 2) 18 bp indel mutation in the 3′-UTR was present in various lengths depending on different monoecious melon germplasm.

Original language	English
Pages (from-to)	535-545
Number of pages	11
Journal	Horticulture Environment and Biotechnology
Volume	56
Issue number	4
DOIs	https://doi.org/10.1007/s13580-015-0024-2
State	Published - 14 Aug 2015

Keywords

cucurbits
flower types
gene-based marker
marker-assisted selection
muskmelon

Access to Document

10.1007/s13580-015-0024-2

Cite this

@article{39f9f40f3a2e48a88c113b0231dcaf98,

title = "The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)",

abstract = "Most melon (Cucumis melo L.) breeding lines in South Korea display andromonoecious sex expression, which necessitates laborious hand emasculation during F1 hybrid seed production. Thus, there is a need to develop monoecious sex types in elite germplasm to obviate self-pollination. Sex expression is associated with floral ethylene production, which, in monecious melon plants, is associated with the A locus. Our study was conducted to develop molecular markers for selection of monoecious plants based on sequence variation inherent in the CmACS-7 gene [encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)] that is associated with ethylene production. Full-length CmACS-7 sequences were cloned from a monoecious (MO23) and an andromonoecious (AM24) line. The alignment of those CmACS-7 sequences revealed a single nucleotide polymorphism (SNP; C170T) in exon 1 and an 18 bp indel in the 3′-untranslated region (UTR) of between MO23 and AM24, which was then used to develop a cleaved amplified polymorphic sequence (CAPS) (EX1-C170T) and a sequence characterized amplified region (SCAR) marker (T1ex), respectively. The sex expression and the T1ex SCAR-based genotype of 442 F2 plants derived from a MO23 × AM24 cross was determined. Monoecy and andromonoecy segregated in a 3:1 ratio in F2 progeny, where the sex type of 429 plants (13 plants not classified) co-segregated with the SCAR marker, demonstrating that sex expression regulated by CmACS-7 is controlled by a single dominant gene and that it confers monoecy in line MO23. Allelic variation in 112 geographically diverse melon lines for CmACS-7 as accessed by CAPS EX1-C170T and SCAR T1ex markers indicated that the: 1) exon 1 of CmACS-7 is highly conserved and the SNP/sex expression association detected is highly predictable making it potentially useful for marker-based selection of monoecious plants, and; 2) 18 bp indel mutation in the 3′-UTR was present in various lengths depending on different monoecious melon germplasm.",

keywords = "cucurbits, flower types, gene-based marker, marker-assisted selection, muskmelon",

author = "Nahui Kim and Juyeol Oh and Bichseam Kim and Choi, {Eung Kyu} and Hwang, {Un Sun} and Staub, {Jack E.} and Chung, {Sang Min} and Younghoon Park",

note = "Publisher Copyright: {\textcopyright} 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH.",

year = "2015",

month = aug,

day = "14",

doi = "10.1007/s13580-015-0024-2",

language = "English",

volume = "56",

pages = "535--545",

journal = "Horticulture Environment and Biotechnology",

issn = "2211-3452",

publisher = "Springer",

number = "4",

}

TY - JOUR

T1 - The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)

AU - Kim, Nahui

AU - Oh, Juyeol

AU - Kim, Bichseam

AU - Choi, Eung Kyu

AU - Hwang, Un Sun

AU - Staub, Jack E.

AU - Chung, Sang Min

AU - Park, Younghoon

PY - 2015/8/14

Y1 - 2015/8/14

N2 - Most melon (Cucumis melo L.) breeding lines in South Korea display andromonoecious sex expression, which necessitates laborious hand emasculation during F1 hybrid seed production. Thus, there is a need to develop monoecious sex types in elite germplasm to obviate self-pollination. Sex expression is associated with floral ethylene production, which, in monecious melon plants, is associated with the A locus. Our study was conducted to develop molecular markers for selection of monoecious plants based on sequence variation inherent in the CmACS-7 gene [encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)] that is associated with ethylene production. Full-length CmACS-7 sequences were cloned from a monoecious (MO23) and an andromonoecious (AM24) line. The alignment of those CmACS-7 sequences revealed a single nucleotide polymorphism (SNP; C170T) in exon 1 and an 18 bp indel in the 3′-untranslated region (UTR) of between MO23 and AM24, which was then used to develop a cleaved amplified polymorphic sequence (CAPS) (EX1-C170T) and a sequence characterized amplified region (SCAR) marker (T1ex), respectively. The sex expression and the T1ex SCAR-based genotype of 442 F2 plants derived from a MO23 × AM24 cross was determined. Monoecy and andromonoecy segregated in a 3:1 ratio in F2 progeny, where the sex type of 429 plants (13 plants not classified) co-segregated with the SCAR marker, demonstrating that sex expression regulated by CmACS-7 is controlled by a single dominant gene and that it confers monoecy in line MO23. Allelic variation in 112 geographically diverse melon lines for CmACS-7 as accessed by CAPS EX1-C170T and SCAR T1ex markers indicated that the: 1) exon 1 of CmACS-7 is highly conserved and the SNP/sex expression association detected is highly predictable making it potentially useful for marker-based selection of monoecious plants, and; 2) 18 bp indel mutation in the 3′-UTR was present in various lengths depending on different monoecious melon germplasm.

AB - Most melon (Cucumis melo L.) breeding lines in South Korea display andromonoecious sex expression, which necessitates laborious hand emasculation during F1 hybrid seed production. Thus, there is a need to develop monoecious sex types in elite germplasm to obviate self-pollination. Sex expression is associated with floral ethylene production, which, in monecious melon plants, is associated with the A locus. Our study was conducted to develop molecular markers for selection of monoecious plants based on sequence variation inherent in the CmACS-7 gene [encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)] that is associated with ethylene production. Full-length CmACS-7 sequences were cloned from a monoecious (MO23) and an andromonoecious (AM24) line. The alignment of those CmACS-7 sequences revealed a single nucleotide polymorphism (SNP; C170T) in exon 1 and an 18 bp indel in the 3′-untranslated region (UTR) of between MO23 and AM24, which was then used to develop a cleaved amplified polymorphic sequence (CAPS) (EX1-C170T) and a sequence characterized amplified region (SCAR) marker (T1ex), respectively. The sex expression and the T1ex SCAR-based genotype of 442 F2 plants derived from a MO23 × AM24 cross was determined. Monoecy and andromonoecy segregated in a 3:1 ratio in F2 progeny, where the sex type of 429 plants (13 plants not classified) co-segregated with the SCAR marker, demonstrating that sex expression regulated by CmACS-7 is controlled by a single dominant gene and that it confers monoecy in line MO23. Allelic variation in 112 geographically diverse melon lines for CmACS-7 as accessed by CAPS EX1-C170T and SCAR T1ex markers indicated that the: 1) exon 1 of CmACS-7 is highly conserved and the SNP/sex expression association detected is highly predictable making it potentially useful for marker-based selection of monoecious plants, and; 2) 18 bp indel mutation in the 3′-UTR was present in various lengths depending on different monoecious melon germplasm.

KW - cucurbits

KW - flower types

KW - gene-based marker

KW - marker-assisted selection

KW - muskmelon

UR - http://www.scopus.com/inward/record.url?scp=84941333514&partnerID=8YFLogxK

U2 - 10.1007/s13580-015-0024-2

DO - 10.1007/s13580-015-0024-2

M3 - Article

AN - SCOPUS:84941333514

SN - 2211-3452

VL - 56

SP - 535

EP - 545

JO - Horticulture Environment and Biotechnology

JF - Horticulture Environment and Biotechnology

IS - 4

ER -

The CmACS-7 gene provides sequence variation for development of DNA markers associated with monoecious sex expression in melon (Cucumis melo L.)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this