TY - JOUR
T1 - The effect of coated nano-hydroxyapatite concentration on scaffolds for osteogenesis
AU - Jang, Hyun Jun
AU - Lee, Eun Cheol
AU - Kwon, Gu Joong
AU - Seo, Young Kwon
N1 - Publisher Copyright:
© The Author(s) 2019.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In this study, we fabricated a silk scaffold containing nano-hydroxyapatite (nano-HAp) for bone tissue engineering applications. The sericin-extracted silk scaffolds were coated with 0.30, 0.15, and 0.03 g of nano-HAp. The scaffolds were soaked in a 1% type I atelocollagen solution and lyophilized. Scaffolds were crosslinked with 0.02% carbodiimide and lyophilized for 48 h, followed by sterilization with γ-irradiation at 10 kGy. The scaffold properties were investigated by energy-dispersive X-ray spectroscopy and atomic force microscope. A typical spectrum of the inorganic crust and the electron diffraction patterns revealed peaks for calcium, phosphorus, and oxygen atoms. Root mean square values of the control and experimental group surfaces were 5.60 and 40.32 nm. The width of nano-HAp was in the approximate range 100–150 nm, and the height was approximately 350 nm. Dental pulp cells were seeded at a density of 2.8 × 104 cells/cm2 and cultured for 3 weeks in a growth medium. The cells were then cultured for 4 weeks in differentiation medium and were transplanted into a nude mouse. The biopsy was processed at 8 weeks. The use of 0.15 g of nano-HAp led to the greatest collagen type III, fibronectin, osteocalcin, osteopontin, osteonectin, osteoprotegerin, and BMP-2 mRNA levels in vitro after 4 weeks in differentiation medium. Western blotting analysis to elucidate signaling pathways was performed. β-Catenin, phosphorylated-ERK, p38 phosphorylation most increased when 0.15 g of nano-HAp was used compared with the control group. In the histological comparison, osteocalcin and osteopontin synthesis were higher for the silk scaffold that contained 0.15 g of nano-HAp. Among the scaffolds, samples containing 0.15 g of nano-HAp were the most effective for osteogenesis. Therefore, this will be a suitable substrate as a biomaterial for bone tissue engineering applications.
AB - In this study, we fabricated a silk scaffold containing nano-hydroxyapatite (nano-HAp) for bone tissue engineering applications. The sericin-extracted silk scaffolds were coated with 0.30, 0.15, and 0.03 g of nano-HAp. The scaffolds were soaked in a 1% type I atelocollagen solution and lyophilized. Scaffolds were crosslinked with 0.02% carbodiimide and lyophilized for 48 h, followed by sterilization with γ-irradiation at 10 kGy. The scaffold properties were investigated by energy-dispersive X-ray spectroscopy and atomic force microscope. A typical spectrum of the inorganic crust and the electron diffraction patterns revealed peaks for calcium, phosphorus, and oxygen atoms. Root mean square values of the control and experimental group surfaces were 5.60 and 40.32 nm. The width of nano-HAp was in the approximate range 100–150 nm, and the height was approximately 350 nm. Dental pulp cells were seeded at a density of 2.8 × 104 cells/cm2 and cultured for 3 weeks in a growth medium. The cells were then cultured for 4 weeks in differentiation medium and were transplanted into a nude mouse. The biopsy was processed at 8 weeks. The use of 0.15 g of nano-HAp led to the greatest collagen type III, fibronectin, osteocalcin, osteopontin, osteonectin, osteoprotegerin, and BMP-2 mRNA levels in vitro after 4 weeks in differentiation medium. Western blotting analysis to elucidate signaling pathways was performed. β-Catenin, phosphorylated-ERK, p38 phosphorylation most increased when 0.15 g of nano-HAp was used compared with the control group. In the histological comparison, osteocalcin and osteopontin synthesis were higher for the silk scaffold that contained 0.15 g of nano-HAp. Among the scaffolds, samples containing 0.15 g of nano-HAp were the most effective for osteogenesis. Therefore, this will be a suitable substrate as a biomaterial for bone tissue engineering applications.
KW - biomaterials
KW - dental pulp stem cell
KW - differentiation
KW - Nano-hydroxyapatite
KW - osteogenesis
UR - http://www.scopus.com/inward/record.url?scp=85074255994&partnerID=8YFLogxK
U2 - 10.1177/0885328219875275
DO - 10.1177/0885328219875275
M3 - Article
C2 - 31526073
AN - SCOPUS:85074255994
SN - 0885-3282
VL - 34
SP - 827
EP - 839
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
IS - 6
ER -