@inbook{64bbb5167be74cf883581965e8ad00f7,
title = "Thermodynamically constrained Flux and Control Analysis of Escherichia coli",
abstract = " Flux Balance Analysis allows calculation of the theoretical maximum yield of desired products given a metabolic network and some experimentally determined fluxes. However these {"} optimal{"} values are often based on thermodynamically infeasible flux distributions. A methodology for the optimisation of fluxes and concentrations subject to thermodynamic constraints is implemented and applied to Escherichia coli to calculate the ranges of possible fluxes and concentrations. The resulting flux bounds together with a random sampling approach to calculate ranges of possible control coefficients, summarised using the control bias concept, are used in this work to indicate potential targets for improving the production of succinic acid with the aid of this organism. The steps with the highest control bias were found to be the glycerol uptake and the steps that bypass the pentose-phosphate pathway. The steps showing strongest negative control were the CO 2 extraction from the system and reactions redirecting pyruvate towards byproducts such as ethanol and formate and away from succinic acid.",
keywords = "Escherichia coli, Flux and Control Analysis, Thermodynamics",
author = "Liliana Angeles-Martineza and Michael Binns and Constantinos Theodoropoulos and {de Atauri}, Pedro and Marta Cascante",
year = "2012",
doi = "10.1016/B978-0-444-59520-1.50134-2",
language = "English",
series = "Computer Aided Chemical Engineering",
publisher = "Elsevier B.V.",
pages = "1377--1381",
booktitle = "Computer Aided Chemical Engineering",
address = "Netherlands",
}