Thiolation of chitosan loaded over super-magnetic halloysite nanotubes for enhanced laccase immobilization

Avinash A. Kadam; Bharat Sharma; Surendra K. Shinde; Gajanan S. Ghodake; Ganesh D. Saratale; Rijuta G. Saratale; Do Yeong Kim; Jung Suk Sung

doi:10.3390/nano10122560

Thiolation of chitosan loaded over super-magnetic halloysite nanotubes for enhanced laccase immobilization

Avinash A. Kadam, Bharat Sharma, Surendra K. Shinde, Gajanan S. Ghodake, Ganesh D. Saratale, Rijuta G. Saratale, Do Yeong Kim, Jung Suk Sung

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18 Scopus citations

Abstract

This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs-Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs-Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs-Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

Original language	English
Article number	2560
Pages (from-to)	1-20
Number of pages	20
Journal	Nanomaterials
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/nano10122560
State	Published - Dec 2020

Keywords

Ampicillin
Direct Red 80
Immobilization
Laccase
Super-magnetic nanosupports for enzyme immobilization
Thiolated chitosan

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10.3390/nano10122560

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@article{c51435f6b6c54313b1d39777af2abd01,

title = "Thiolation of chitosan loaded over super-magnetic halloysite nanotubes for enhanced laccase immobilization",

abstract = "This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs-Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs-Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs-Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.",

keywords = "Ampicillin, Direct Red 80, Immobilization, Laccase, Super-magnetic nanosupports for enzyme immobilization, Thiolated chitosan",

author = "Kadam, {Avinash A.} and Bharat Sharma and Shinde, {Surendra K.} and Ghodake, {Gajanan S.} and Saratale, {Ganesh D.} and Saratale, {Rijuta G.} and Kim, {Do Yeong} and Sung, {Jung Suk}",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = dec,

doi = "10.3390/nano10122560",

language = "English",

volume = "10",

pages = "1--20",

journal = "Nanomaterials",

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TY - JOUR

T1 - Thiolation of chitosan loaded over super-magnetic halloysite nanotubes for enhanced laccase immobilization

AU - Kadam, Avinash A.

AU - Sharma, Bharat

AU - Shinde, Surendra K.

AU - Ghodake, Gajanan S.

AU - Saratale, Ganesh D.

AU - Saratale, Rijuta G.

AU - Kim, Do Yeong

AU - Sung, Jung Suk

PY - 2020/12

Y1 - 2020/12

N2 - This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs-Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs-Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs-Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

AB - This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs-Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs-Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs-Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

KW - Ampicillin

KW - Direct Red 80

KW - Immobilization

KW - Laccase

KW - Super-magnetic nanosupports for enzyme immobilization

KW - Thiolated chitosan

UR - http://www.scopus.com/inward/record.url?scp=85098137323&partnerID=8YFLogxK

U2 - 10.3390/nano10122560

DO - 10.3390/nano10122560

M3 - Article

AN - SCOPUS:85098137323

SN - 2079-4991

VL - 10

SP - 1

EP - 20

JO - Nanomaterials

JF - Nanomaterials

IS - 12

M1 - 2560

ER -

Thiolation of chitosan loaded over super-magnetic halloysite nanotubes for enhanced laccase immobilization

Abstract

Keywords

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