Transcriptome analysis in response to UV-B stress in soybean [Glycine max (L.) Merr]

Jun Cheol Moon, Won Cheol Yim, Jae Eun Lee, Young Up Kwon, Kitae Song, Byung Moo Lee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In order to identify the genes specifically or predominantly expressed in UV-B illuminated soybean [Glycine max (L.) Merr.] leaves, this study compared the differential expression levels of the mRNA fragments of untreated leaves and UV-B treated leaves using differential banding patterns in agarose gels. In the UV-B illuminated soybean leaves, the differentially expressed band patterns were determined to be 207 up-regulated bands and 214 down-regulated bands. A total of 356 differentially expressed genes (DEGs) in the UV-B illuminated soybean leaves were sequenced. Using BLASTx, the putative functions of the expressed sequence tags were identified. Most of the identified UV-B-responsive genes related to other biotic and abiotic stresses. The characterized ESTs will provide useful data in developing our understanding of the molecular bases and transcript profiles of UV-B responses.

Original languageEnglish
Pages (from-to)1395-1400
Number of pages6
JournalAustralian Journal of Crop Science
Volume6
Issue number9
StatePublished - Sep 2012

Keywords

  • Differentially expressed genes
  • Soybean
  • Transcript
  • UV-B stress

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