Abstract
In order to identify the genes specifically or predominantly expressed in UV-B illuminated soybean [Glycine max (L.) Merr.] leaves, this study compared the differential expression levels of the mRNA fragments of untreated leaves and UV-B treated leaves using differential banding patterns in agarose gels. In the UV-B illuminated soybean leaves, the differentially expressed band patterns were determined to be 207 up-regulated bands and 214 down-regulated bands. A total of 356 differentially expressed genes (DEGs) in the UV-B illuminated soybean leaves were sequenced. Using BLASTx, the putative functions of the expressed sequence tags were identified. Most of the identified UV-B-responsive genes related to other biotic and abiotic stresses. The characterized ESTs will provide useful data in developing our understanding of the molecular bases and transcript profiles of UV-B responses.
Original language | English |
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Pages (from-to) | 1395-1400 |
Number of pages | 6 |
Journal | Australian Journal of Crop Science |
Volume | 6 |
Issue number | 9 |
State | Published - Sep 2012 |
Keywords
- Differentially expressed genes
- Soybean
- Transcript
- UV-B stress