Translation of a histone H3 tail as a model system for studying peptidyl-tRNA drop-off

Taek Jin Kang; Hiroaki Suga

doi:10.1016/j.febslet.2011.05.051

Translation of a histone H3 tail as a model system for studying peptidyl-tRNA drop-off

Taek Jin Kang, Hiroaki Suga

Department of Chemical and Biochemical Engineering

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We found that the synthesis of histone H3 N-terminal peptide (tail) in a reconstituted protein synthesis system yielded fragmented peptides along with the full-length product. With the combined use of MALDI-TOF analysis and peptidyl-tRNA hydrolase cleavage of the Flag tagged product species, we concluded that the fragments were generated by peptidyl-tRNA drop-off at specific sites and subsequent translation continuation. Using the histone H3 tail we also found that peptidyl-tRNA drop-off is strongly correlated with the amino acid context. We envision that the system described here would be useful as a model system for studying peptidyl-tRNA drop-off events.

Original language	English
Pages (from-to)	2269-2274
Number of pages	6
Journal	FEBS Letters
Volume	585
Issue number	14
DOIs	https://doi.org/10.1016/j.febslet.2011.05.051
State	Published - 21 Jul 2011

Keywords

Peptidyl-tRNA drop-off
Peptidyl-tRNA hydrolase
Translation

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10.1016/j.febslet.2011.05.051

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abstract = "We found that the synthesis of histone H3 N-terminal peptide (tail) in a reconstituted protein synthesis system yielded fragmented peptides along with the full-length product. With the combined use of MALDI-TOF analysis and peptidyl-tRNA hydrolase cleavage of the Flag tagged product species, we concluded that the fragments were generated by peptidyl-tRNA drop-off at specific sites and subsequent translation continuation. Using the histone H3 tail we also found that peptidyl-tRNA drop-off is strongly correlated with the amino acid context. We envision that the system described here would be useful as a model system for studying peptidyl-tRNA drop-off events.",

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AB - We found that the synthesis of histone H3 N-terminal peptide (tail) in a reconstituted protein synthesis system yielded fragmented peptides along with the full-length product. With the combined use of MALDI-TOF analysis and peptidyl-tRNA hydrolase cleavage of the Flag tagged product species, we concluded that the fragments were generated by peptidyl-tRNA drop-off at specific sites and subsequent translation continuation. Using the histone H3 tail we also found that peptidyl-tRNA drop-off is strongly correlated with the amino acid context. We envision that the system described here would be useful as a model system for studying peptidyl-tRNA drop-off events.

KW - Peptidyl-tRNA drop-off

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Translation of a histone H3 tail as a model system for studying peptidyl-tRNA drop-off

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