TY - JOUR
T1 - Tumebacillus amylolyticus sp. nov., isolated from garden soil in Korea
AU - Kang, Minchung
AU - Chhetri, Geeta
AU - Kim, Jiyoun
AU - Kim, Inhyup
AU - So, Yoonseop
AU - Seo, Taegun
N1 - Publisher Copyright:
© 2022 The Authors.Microbiology Society. All right reserved.
PY - 2022
Y1 - 2022
N2 - A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea. The strain was identified as Gram-stain-positive, rod-shaped, and motile by means of peritrichous flagella. Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3%) while also clustering with Tumebacillus flagellatus GST4T (97.9%). Strain ITR2T grew optimally at temperatures of 20–30 °C, at pH 6.0–7.0 and at NaCl concentrations of 0–1%. The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan). The major fatty acids (>10%) of the novel strain were C16:0, iso-C14:0, iso-C15:0 and anteiso-C15:0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphati-dylglycerol and one unidentified phospholipid. Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid. The in silico DNA–DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9%, respectively, while the average nucleotide identity values were 78.5 and 81.2%, respectively. The genomic DNA G+C content was 54.9mol%. Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp. nov. to be a novel species within the genus Tumebacillus. The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
AB - A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea. The strain was identified as Gram-stain-positive, rod-shaped, and motile by means of peritrichous flagella. Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3%) while also clustering with Tumebacillus flagellatus GST4T (97.9%). Strain ITR2T grew optimally at temperatures of 20–30 °C, at pH 6.0–7.0 and at NaCl concentrations of 0–1%. The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan). The major fatty acids (>10%) of the novel strain were C16:0, iso-C14:0, iso-C15:0 and anteiso-C15:0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphati-dylglycerol and one unidentified phospholipid. Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid. The in silico DNA–DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9%, respectively, while the average nucleotide identity values were 78.5 and 81.2%, respectively. The genomic DNA G+C content was 54.9mol%. Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp. nov. to be a novel species within the genus Tumebacillus. The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
KW - amylolytic bacteria
KW - phylogenetic analysis
KW - starch
KW - starch-degrading
KW - Tumebacillus
UR - http://www.scopus.com/inward/record.url?scp=85130188511&partnerID=8YFLogxK
U2 - 10.1099/IJSEM.0.005376
DO - 10.1099/IJSEM.0.005376
M3 - Article
C2 - 35580016
AN - SCOPUS:85130188511
SN - 1466-5026
VL - 72
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 5
M1 - 005376
ER -