Abstract
In this work, it was discovered that the stability of mRNA in a cell-free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequentially. As a result of the controlled abundance of mRNA species, it was possible to manipulate the relative expression level of target proteins by employing the T7 terminator of adjusted stem lengths.
Original language | English |
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Pages (from-to) | 422-427 |
Number of pages | 6 |
Journal | Biotechnology and Bioengineering |
Volume | 101 |
Issue number | 2 |
DOIs | |
State | Published - 1 Oct 2008 |
Keywords
- Cell-free protein synthesis
- Metabolic engineering
- mRNA stability
- Synthetic biology
- T7 terminator
- Tunable expression