Tuning the expression level of recombinant proteins by modulating mRNA stability in a cell-free protein synthesis system

Jin Ho Ahn, Taek Jin Kang, Dong Myung Kim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

In this work, it was discovered that the stability of mRNA in a cell-free extract could be controlled by using engineered T7 terminator sequences. Specifically, it was found that mRNA stability gradually decreased as the length of the stem structure of the T7 terminator was reduced sequentially. As a result of the controlled abundance of mRNA species, it was possible to manipulate the relative expression level of target proteins by employing the T7 terminator of adjusted stem lengths.

Original languageEnglish
Pages (from-to)422-427
Number of pages6
JournalBiotechnology and Bioengineering
Volume101
Issue number2
DOIs
StatePublished - 1 Oct 2008

Keywords

  • Cell-free protein synthesis
  • Metabolic engineering
  • mRNA stability
  • Synthetic biology
  • T7 terminator
  • Tunable expression

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