Type-specific viral load and physical state of HPV Type 16, 18, and 58 as diagnostic biomarkers for high-grade squamous intraepithelial lesions or cervical cancer

Jongseung Kim, Bu Kyung Kim, Dongsoo Jeon, Chae Hyeong Lee, Ju Won Roh, Joo Young Kim, Sang Yoon Park

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Purpose High rate of false-positive tests is a major obstacle to use human papillomavirus (HPV) detection as a diagnostic tool for high-grade squamous intraepithelial lesions or cervical cancer (HSIL+). We investigated whether type-specific viral load or physical state of HPV 16, 18, and 58 are useful biomarkers for HSIL+. Materials and Methods Type-specific viral loads of E6 and E2 genes in cervical cells from 240, 83, and 79 HPV 16 , 18 , and 58 infected women, respectively, were determined using real-time polymerase chain reaction. Viral loads were normalized to cellular DNA (copy/cell). Total and integrated viral loads and physical state were compared between HSIL+ and controls, and diagnostic value was determined using receiver operating characteristic analysis. Results Viral loads of HPV 16, 18, and 58 were significantly different in lesions in the same pathologic grade. High type-specific total viral loads were significantly associated with HSIL+ (odds ratio [OR], 14.065, 39.472, and 7.103 for HPV 16, 18, and 58, respectively). High integrated viral load was related to HSIL+ in women with HPV 16 (OR, 8.242), and integrated state was associated with HSIL+ in women with HPV 18 (OR, 9.443). Type-specific total viral load was significantly associated with HSIL+ (area under curve, 0.914, 0.937, and 0.971 for HPV 16, 18, and 58, respectively), indicating an excellent performance in detecting HSIL+. Conclusion Type-specific total viral load may be a powerful diagnostic marker for HSIL+ in HPV 16 , 18 , and 58 infected HSIL+ lesions. If demonstrated in all other high-risk HPV types, this method can lead to a paradigm shift in the strategy of equivocal cytologic abnormalities.

Original languageEnglish
Pages (from-to)396-405
Number of pages10
JournalCancer Research and Treatment
Volume52
Issue number2
DOIs
StatePublished - 2020

Keywords

  • Cervical cancer
  • High-grade squamous intraepithelial lesions
  • Human papillomavirus
  • Viral load

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