Ultrasound-triggered three dimensional hyaluronic acid hydrogel promotes in vitro and in vivo reprogramming into induced pluripotent stem cells

Deogil Kim, Min Ju Lee, Yoshie Arai, Jinsung Ahn, Gun Woo Lee, Soo Hong Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Cellular reprogramming technologies have been developed with different physicochemical factors to improve the reprogramming efficiencies of induced pluripotent stem cells (iPSCs). Ultrasound is a clinically applied noncontact biophysical factor known for regulating various cellular behaviors but remains uninvestigated for cellular reprogramming. Here, we present a new reprogramming strategy using low-intensity ultrasound (LIUS) to improve cellular reprogramming of iPSCs in vitro and in vivo. Under 3D microenvironment conditions, increased LIUS stimulation shows enhanced cellular reprogramming of the iPSCs. The cellular reprogramming process facilitated by LIUS is accompanied by increased mesenchymal to epithelial transition and histone modification. LIUS stimulation transiently modulates the cytoskeletal rearrangement, along with increased membrane fluidity and mobility to increase HA/CD44 interactions. Furthermore, LIUS stimulation with HA hydrogel can be utilized in application of both human cells and in vivo environment, for enhanced reprogrammed cells into iPSCs. Thus, LIUS stimulation with a combinatorial 3D microenvironment system can improve cellular reprogramming in vitro and in vivo environments, which can be applied in various biomedical fields.

Original languageEnglish
Pages (from-to)331-345
Number of pages15
JournalBioactive Materials
Volume38
DOIs
StatePublished - Aug 2024

Keywords

  • Biophysical stimulation
  • Cellular reprogramming
  • Cytoskeletal rearrangement
  • Induced pluripotent stem cell
  • Low-intensity ultrasound

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