User-friendly universal and durable subcellular-scaled template for protein binding: Application to single-cell patterning

Jae Won Jang; John M. Collins; Saju Nettikadan

doi:10.1002/adfm.201301088

User-friendly universal and durable subcellular-scaled template for protein binding: Application to single-cell patterning

Jae Won Jang, John M. Collins, Saju Nettikadan

Division of System Semiconductor

NanoInk, Inc.

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A new method for subcellular-sized protein patterning on a SiOx substrate is demonstrated by dip-pen nanolithography printed aldehyde-terminated alkylsilane template. The aldehyde-silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using one-year old aldehyde-silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde-silane template. With more than half of chance, single- or double-cells are successfully attached on fibronectin protein nanoarrays in 21 × 21 μm ² (7 × 7 dot array) and 42 × 42 μm² (14 × 14 dot array). The fibronectin nanoarray with small area (21 × 21 μm²) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials.

Original language	English
Pages (from-to)	5840-5845
Number of pages	6
Journal	Advanced Functional Materials
Volume	23
Issue number	47
DOIs	https://doi.org/10.1002/adfm.201301088
State	Published - 17 Dec 2013

Keywords

aldehyde-amine reaction
cell patterning
dip-pen nanolithography
nanostructures
protein patterning

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10.1002/adfm.201301088

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title = "User-friendly universal and durable subcellular-scaled template for protein binding: Application to single-cell patterning",

abstract = "A new method for subcellular-sized protein patterning on a SiOx substrate is demonstrated by dip-pen nanolithography printed aldehyde-terminated alkylsilane template. The aldehyde-silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using one-year old aldehyde-silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde-silane template. With more than half of chance, single- or double-cells are successfully attached on fibronectin protein nanoarrays in 21 × 21 μm 2 (7 × 7 dot array) and 42 × 42 μm2 (14 × 14 dot array). The fibronectin nanoarray with small area (21 × 21 μm2) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials.",

keywords = "aldehyde-amine reaction, cell patterning, dip-pen nanolithography, nanostructures, protein patterning",

author = "Jang, {Jae Won} and Collins, {John M.} and Saju Nettikadan",

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T1 - User-friendly universal and durable subcellular-scaled template for protein binding

T2 - Application to single-cell patterning

AU - Jang, Jae Won

AU - Collins, John M.

AU - Nettikadan, Saju

PY - 2013/12/17

Y1 - 2013/12/17

N2 - A new method for subcellular-sized protein patterning on a SiOx substrate is demonstrated by dip-pen nanolithography printed aldehyde-terminated alkylsilane template. The aldehyde-silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using one-year old aldehyde-silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde-silane template. With more than half of chance, single- or double-cells are successfully attached on fibronectin protein nanoarrays in 21 × 21 μm 2 (7 × 7 dot array) and 42 × 42 μm2 (14 × 14 dot array). The fibronectin nanoarray with small area (21 × 21 μm2) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials.

AB - A new method for subcellular-sized protein patterning on a SiOx substrate is demonstrated by dip-pen nanolithography printed aldehyde-terminated alkylsilane template. The aldehyde-silane template is stable and durable; for example, subcellular scaled IgG protein array can be obtained using one-year old aldehyde-silane template. Moreover, single cell patterning is successfully carried out by extracellular material (ECM) protein microarray and nanoarray fabricated on an aldehyde-silane template. With more than half of chance, single- or double-cells are successfully attached on fibronectin protein nanoarrays in 21 × 21 μm 2 (7 × 7 dot array) and 42 × 42 μm2 (14 × 14 dot array). The fibronectin nanoarray with small area (21 × 21 μm2) shows the more rate of single cell attachment. Therefore, it is also demonstrated that cell patterning can be controlled by adjusting the nanostructure of ECM materials.

KW - aldehyde-amine reaction

KW - cell patterning

KW - dip-pen nanolithography

KW - nanostructures

KW - protein patterning

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DO - 10.1002/adfm.201301088

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JO - Advanced Functional Materials

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ER -

User-friendly universal and durable subcellular-scaled template for protein binding: Application to single-cell patterning

Abstract

Keywords

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